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- W1991656613 abstract "Luminescence catalyst immunoassay, a nonradioactive and nonenzymatic immunoassay, has been applied to the determination of β2-microglobulin (β2-MG). The method is characterized by high sensitivity and simple handling, since the analytical method employs a catalytically amplifiable label and solid-phase sandwich technique, respectively. In the first stage, an antibody-immobilized plate is reacted with the analyte (β2-MG). In the second stage, the bound β2-MG undergoes successive binding with haemin-labelled antibody. In the last stage, the plate is placed in the luminol-H2O2 system to generate luminescence. A calibration curve with a β2-MG concentration at the midpoint of 100 ng ml−1 was obtained. The 10 and 90% response levels in the curve are 10 and 1000 ng ml−1." @default.
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- W1991656613 date "1983-05-01" @default.
- W1991656613 modified "2023-09-24" @default.
- W1991656613 title "Luminescence catalyst immunoassay of β2-microglobulin with haemin as a chemically amplifiable label" @default.
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- W1991656613 doi "https://doi.org/10.1016/0141-0229(83)90098-4" @default.
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