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- W1991706449 abstract "Abstract A new method for separation and determination of amygdalin and its epimer (neoamygdalin) in the epimerization of amygdalin by MEEKC is proposed. For the chiral separation of amygdalin and neoamygdalin, a running buffer composed of 80 mM sodium cholate, 5.0% v/v butan‐1‐ol, 0.5% v/v heptane and 94.5% v/v 30 mM Na 2 B 4 O 7 buffer (pH 9.00) is proposed. Under optimum conditions, the basic separation of amygdalin and neoamygdalin can be achieved within 7 min. The calibration curve for amygdalin showed excellent linearity in the concentration range of 20–1000 μg/mL with a detection limit of 5.0 μg/mL (S/N=3). The epimerization rate constant of amygdalin in basic microemulsion was first determined by monitoring the concentration changes of amygdalin, and the epimerization rate constant of amygdalin was found to be 2×10 −3 min −1 at 25°C under the above optimum microemulsion conditions." @default.
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- W1991706449 date "2010-12-17" @default.
- W1991706449 modified "2023-10-18" @default.
- W1991706449 title "Determination of the epimerization rate constant of amygdalin by microemulsion electrokinetic chromatography" @default.
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- W1991706449 doi "https://doi.org/10.1002/elps.201000287" @default.
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