Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991736235> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1991736235 endingPage "74" @default.
- W1991736235 startingPage "68" @default.
- W1991736235 abstract "A parallel chromatographic procedure for the purification of milligram amounts of plasmid DNA was developed. Initial studies showed that ion-exchange membrane capsules displayed high capacity for plasmid DNA. Interestingly, a weak anion exchanger (DEAE) proved to be superior to the strong quarternary ammonium group with respect to elution and regeneration properties and the 75 cm(2) Sartobind D membrane capsule (MA75D, Sartorius) was selected for further studies. A method for reducing endotoxin levels by using CTAB as a precipitant was optimised. By introducing this step into the protocol, endotoxin levels could be reduced approximately 100-fold to <or=5 EU/mg plasmid. The parallel procedure was set up on a multi-channel peristaltic pump and evaluated with four different vectors (2.7-11.5 kbp). Starting with 5-10 g of E. coli cell paste (wet weight) generally saturated the membrane adsorber, resulting in plasmid DNA yields close to 10 mg." @default.
- W1991736235 created "2016-06-24" @default.
- W1991736235 creator A5008822387 @default.
- W1991736235 creator A5012631421 @default.
- W1991736235 creator A5042728325 @default.
- W1991736235 creator A5077150372 @default.
- W1991736235 date "2007-09-01" @default.
- W1991736235 modified "2023-10-14" @default.
- W1991736235 title "Milligram scale parallel purification of plasmid DNA using anion-exchange membrane capsules and a multi-channel peristaltic pump" @default.
- W1991736235 cites W129129599 @default.
- W1991736235 cites W1850391599 @default.
- W1991736235 cites W1968334024 @default.
- W1991736235 cites W1979431132 @default.
- W1991736235 cites W1986247744 @default.
- W1991736235 cites W200261047 @default.
- W1991736235 cites W2003724638 @default.
- W1991736235 cites W2015242294 @default.
- W1991736235 cites W2019410656 @default.
- W1991736235 cites W2028858374 @default.
- W1991736235 cites W2033604473 @default.
- W1991736235 cites W2092786524 @default.
- W1991736235 cites W2133889068 @default.
- W1991736235 cites W4293247451 @default.
- W1991736235 doi "https://doi.org/10.1016/j.jchromb.2007.05.033" @default.
- W1991736235 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17604704" @default.
- W1991736235 hasPublicationYear "2007" @default.
- W1991736235 type Work @default.
- W1991736235 sameAs 1991736235 @default.
- W1991736235 citedByCount "12" @default.
- W1991736235 countsByYear W19917362352013 @default.
- W1991736235 countsByYear W19917362352018 @default.
- W1991736235 countsByYear W19917362352023 @default.
- W1991736235 crossrefType "journal-article" @default.
- W1991736235 hasAuthorship W1991736235A5008822387 @default.
- W1991736235 hasAuthorship W1991736235A5012631421 @default.
- W1991736235 hasAuthorship W1991736235A5042728325 @default.
- W1991736235 hasAuthorship W1991736235A5077150372 @default.
- W1991736235 hasConcept C117760992 @default.
- W1991736235 hasConcept C121332964 @default.
- W1991736235 hasConcept C134519083 @default.
- W1991736235 hasConcept C145148216 @default.
- W1991736235 hasConcept C153294291 @default.
- W1991736235 hasConcept C178790620 @default.
- W1991736235 hasConcept C185592680 @default.
- W1991736235 hasConcept C22744801 @default.
- W1991736235 hasConcept C2778778583 @default.
- W1991736235 hasConcept C2779870022 @default.
- W1991736235 hasConcept C41625074 @default.
- W1991736235 hasConcept C43617362 @default.
- W1991736235 hasConcept C552990157 @default.
- W1991736235 hasConcept C55493867 @default.
- W1991736235 hasConcept C59822182 @default.
- W1991736235 hasConcept C73826308 @default.
- W1991736235 hasConcept C86803240 @default.
- W1991736235 hasConceptScore W1991736235C117760992 @default.
- W1991736235 hasConceptScore W1991736235C121332964 @default.
- W1991736235 hasConceptScore W1991736235C134519083 @default.
- W1991736235 hasConceptScore W1991736235C145148216 @default.
- W1991736235 hasConceptScore W1991736235C153294291 @default.
- W1991736235 hasConceptScore W1991736235C178790620 @default.
- W1991736235 hasConceptScore W1991736235C185592680 @default.
- W1991736235 hasConceptScore W1991736235C22744801 @default.
- W1991736235 hasConceptScore W1991736235C2778778583 @default.
- W1991736235 hasConceptScore W1991736235C2779870022 @default.
- W1991736235 hasConceptScore W1991736235C41625074 @default.
- W1991736235 hasConceptScore W1991736235C43617362 @default.
- W1991736235 hasConceptScore W1991736235C552990157 @default.
- W1991736235 hasConceptScore W1991736235C55493867 @default.
- W1991736235 hasConceptScore W1991736235C59822182 @default.
- W1991736235 hasConceptScore W1991736235C73826308 @default.
- W1991736235 hasConceptScore W1991736235C86803240 @default.
- W1991736235 hasIssue "1-2" @default.
- W1991736235 hasLocation W19917362351 @default.
- W1991736235 hasLocation W19917362352 @default.
- W1991736235 hasOpenAccess W1991736235 @default.
- W1991736235 hasPrimaryLocation W19917362351 @default.
- W1991736235 hasRelatedWork W1974950787 @default.
- W1991736235 hasRelatedWork W2111187553 @default.
- W1991736235 hasRelatedWork W2117422820 @default.
- W1991736235 hasRelatedWork W2124622445 @default.
- W1991736235 hasRelatedWork W2186603117 @default.
- W1991736235 hasRelatedWork W2357652707 @default.
- W1991736235 hasRelatedWork W2358162314 @default.
- W1991736235 hasRelatedWork W2365313273 @default.
- W1991736235 hasRelatedWork W2625992620 @default.
- W1991736235 hasRelatedWork W3193051939 @default.
- W1991736235 hasVolume "856" @default.
- W1991736235 isParatext "false" @default.
- W1991736235 isRetracted "false" @default.
- W1991736235 magId "1991736235" @default.
- W1991736235 workType "article" @default.