Matches in SemOpenAlex for { <https://semopenalex.org/work/W199177544> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W199177544 abstract "The ribosomal DNA fingerprints of three strains of harmful algae, genus Pseudo-nitzschia designated PNA, PNB and PNC were examined. Harmful algae samples were collectedfrom the coastal area of Sepanggar Bay, Sabah. The samples collected were cultured in suspension culture using L1 medium and the algae growth rate were monitored by doingcell counting. PNA reached the log phase after day 13 of culture, while PNB and PNC on day 14 and 16 respectively. Although PNC reached the log phase slower than the othertwo samples, it recorded more cells during the log phase compared to the other two. When the algae growth rate reached the log phase, DNA was extracted from each of thesamples and amplified using primers targeting the small subunit of the rDNA through Polymerase Chain Reaction (PCR). Two PCR products with the size of 1,280 bp and 900bp were obtained. Subsequently, the PCR products were subjected to restriction enzyme digestions by using Haelll. The digested DNA fragments of the three strains of samplesshowed that there were genetic differences among the three samples based on the number and size of digested fragments obtained. Four digested DNA fragments were observed inPNA and PNB while six digested fragments were observed in PNC. Degree of similarity calculated according to the Nei & Li (1979) similarity index showed that the degree ofsimilarity between PNA and PNB was 1 and their genetic distance was 0. While the degree of similarity between PNC and these two strains was 0.8 and their genetic distancewas 0.2. This showed that PNA and PNB were from the same strain, while PNC was a different strain from PNA and PNB. As a conclusion, in this study genetic molecular technique was found to be useful in identifying differences between algae strains." @default.
- W199177544 created "2016-06-24" @default.
- W199177544 creator A5072453888 @default.
- W199177544 date "2005-01-01" @default.
- W199177544 modified "2023-09-26" @default.
- W199177544 title "Ribosomal DNA fingerprinting of diatom, genus Pseudo-nitzschia" @default.
- W199177544 hasPublicationYear "2005" @default.
- W199177544 type Work @default.
- W199177544 sameAs 199177544 @default.
- W199177544 citedByCount "0" @default.
- W199177544 crossrefType "journal-article" @default.
- W199177544 hasAuthorship W199177544A5072453888 @default.
- W199177544 hasConcept C104317684 @default.
- W199177544 hasConcept C128319531 @default.
- W199177544 hasConcept C153911025 @default.
- W199177544 hasConcept C207812311 @default.
- W199177544 hasConcept C2778902744 @default.
- W199177544 hasConcept C49105822 @default.
- W199177544 hasConcept C54355233 @default.
- W199177544 hasConcept C552990157 @default.
- W199177544 hasConcept C559758991 @default.
- W199177544 hasConcept C59822182 @default.
- W199177544 hasConcept C67905577 @default.
- W199177544 hasConcept C79386861 @default.
- W199177544 hasConcept C86803240 @default.
- W199177544 hasConcept C90132467 @default.
- W199177544 hasConceptScore W199177544C104317684 @default.
- W199177544 hasConceptScore W199177544C128319531 @default.
- W199177544 hasConceptScore W199177544C153911025 @default.
- W199177544 hasConceptScore W199177544C207812311 @default.
- W199177544 hasConceptScore W199177544C2778902744 @default.
- W199177544 hasConceptScore W199177544C49105822 @default.
- W199177544 hasConceptScore W199177544C54355233 @default.
- W199177544 hasConceptScore W199177544C552990157 @default.
- W199177544 hasConceptScore W199177544C559758991 @default.
- W199177544 hasConceptScore W199177544C59822182 @default.
- W199177544 hasConceptScore W199177544C67905577 @default.
- W199177544 hasConceptScore W199177544C79386861 @default.
- W199177544 hasConceptScore W199177544C86803240 @default.
- W199177544 hasConceptScore W199177544C90132467 @default.
- W199177544 hasLocation W1991775441 @default.
- W199177544 hasOpenAccess W199177544 @default.
- W199177544 hasPrimaryLocation W1991775441 @default.
- W199177544 hasRelatedWork W1250066923 @default.
- W199177544 hasRelatedWork W1599238298 @default.
- W199177544 hasRelatedWork W1965116332 @default.
- W199177544 hasRelatedWork W1970236900 @default.
- W199177544 hasRelatedWork W2006329913 @default.
- W199177544 hasRelatedWork W2011363158 @default.
- W199177544 hasRelatedWork W2061853296 @default.
- W199177544 hasRelatedWork W2095559623 @default.
- W199177544 hasRelatedWork W2105320976 @default.
- W199177544 hasRelatedWork W2105620360 @default.
- W199177544 hasRelatedWork W2107853781 @default.
- W199177544 hasRelatedWork W2109439333 @default.
- W199177544 hasRelatedWork W2151303870 @default.
- W199177544 hasRelatedWork W2171533830 @default.
- W199177544 hasRelatedWork W2357630302 @default.
- W199177544 hasRelatedWork W2360383374 @default.
- W199177544 hasRelatedWork W834523711 @default.
- W199177544 hasRelatedWork W92072545 @default.
- W199177544 hasRelatedWork W1235190359 @default.
- W199177544 hasRelatedWork W2185440809 @default.
- W199177544 isParatext "false" @default.
- W199177544 isRetracted "false" @default.
- W199177544 magId "199177544" @default.
- W199177544 workType "article" @default.