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- W1991786578 abstract "Noninvasive examination of embryo metabolism can be accomplished using metabolomics technology to analyze media following in vitro culture. The objective of this study was to characterize embryo metabolites that are related to blastocyst viability. Research study. Compact morulae (B6D2F1 mice) were recovered on d2.5 following ovarian stimulation and mating (in vivo), or were produced following IVM/IVF/IVC (78 hpi; in vitro). Embryos were cultured individually in 7 μL medium for 24 hr to expanded blastocyst. Media collected from drops that did and did not contain embryos was analyzed using GC-MS and MALDI-MS (n=23). Of the 30 identified metabolites detected in culture media, 13 were significantly different between media in which in vivo versus in vitro embryos had been cultured. In vitro produced embryos were more metabolically active, depleting greater amounts of asparagine, aspartate, cystine, glucitol, glucose, isoleucine, lactate, leucine, lysine, ornithine, phenylalanine, phosphoric acid and tyrosine from the media during culture. In vitro embryos with low Oct4 expression took up significantly more aspartate than in vivo embryos or in vitro embryos with high Oct4 expression. In contrast to published reports, lactate was not produced but tended to be taken up by in vitro embryos and was unchanged by in vivo embryos. Glucose was not significantly taken up by in vivo embryos, although it was by in vitro embryos. Two contaminants of culture medium were also discovered (ditertbutylphenol and an unknown compound). Reduced metabolite use is associated with higher quality (in vivo) embryos. This study demonstrates that metabolomic technology can non-invasively detect differences in metabolism between embryos of different quality, and provides a novel look at embryo metabolism in a complex media environment. In particular, aspartate may be a useful biomarker to predict embryo viability." @default.
- W1991786578 created "2016-06-24" @default.
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- W1991786578 date "2013-09-01" @default.
- W1991786578 modified "2023-09-26" @default.
- W1991786578 title "Metabolic differences between in vivo and in vitro produced embryos suggest critical metabolites related to viability" @default.
- W1991786578 doi "https://doi.org/10.1016/j.fertnstert.2013.07.1227" @default.
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