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- W1991788900 abstract "1. The muscular parvalbumins present in carp (Cyprinus carpio) myogen have been isolated simultaneously by a three-step procedure involving (NH4)2SO4 fractionation, gel filtration on Sephadex G-75 and chromatography on DEAE cellulose. 2. Disc electrophoresis in 12% polyacrylamide gels was also found useful in analytically separating these parvalbumins, either from whole myogen or at different stages of their purification. 3. The low-molecular-weight fraction of carp myogen was submitted to isoelectric focusing in a sucrose gradient, permitting the isoelectric points of the four major parvalbumins to be determined as being 4.47, 4.37, 4.25 and 3.95 at 2°. 4. The three techniques above were found to be applicable to the isolation and characterization of the parvalbumins from hake (Merluccius merluccius), turbot (Psetta maxima) and frog (Rana esculenta). 5. Their general use in the study of muscular parvalbumins is discussed." @default.
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- W1991788900 date "1971-05-01" @default.
- W1991788900 modified "2023-09-28" @default.
- W1991788900 title "Muscular parvalbumins: Preparative and analytical methods of general applicability" @default.
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- W1991788900 doi "https://doi.org/10.1016/0005-2795(71)90220-0" @default.
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