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- W1991790077 abstract "A brush border membrane enriched fraction was isolated from human, full-term placenta. This membrane fraction exhibited large membrane fragments with microvilli projecting from the basal membrane in electron micrographs and was enriched tenfold in alkaline phosphatase, a brush border enzyme marker. The sialoglycoproteins associated with this membrane fraction were tritiated by mild periodate oxidation of sialic acid and reduction with tritiated NaBH4. The membranes were solubilized in 8 M urea, 2% Triton X-100, and the tritiated glycoprotein subunits were reduced with β-mercaptoethanol and characterized by 2-dimensional polyacrylamide gel electrophoresis using a method similar to that described by O'Farrell and Bhakdi, Knüferman, and Wallach. The tritiated subunits were detected in the gels by autofluorography. The 2-dimensional subunit “maps” resolved at least 17 major sialoglycoprotein subunits whereas only 10 major periodate-Schiff reagent staining components were resolved by 1-dimensional SDS polyacrylamide gel electrophoresis. Placental alkaline phosphatase (PAP) was identified on the subunit maps by inclusion of 32P-labeled PAP in the tritiated membrane sample. The 32P-labeled PAP corresponded to a major tritiated sialoglycoprotein subunit, which was heterogeneous with respect to charge as demonstrated by 3 closely running spots of the same molecular weight." @default.
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- W1991790077 date "1977-01-01" @default.
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- W1991790077 title "The sialoglycoprotein subunits of human placental brush border membranes characterized by two-dimensional electrophoresis" @default.
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- W1991790077 doi "https://doi.org/10.1002/jss.400060402" @default.
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