FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991799302> ?p ?o ?g. }

• W1991799302 endingPage "708" @default.
• W1991799302 startingPage "698" @default.
• W1991799302 abstract "1. In the human airway epithelium, VIP/PACAP receptors are distributed in nerve fibers and in epithelial cells but their role in transepithelial ion transport have not been reported. Here, we show that human bronchial epithelial Calu-3 cells expressed the VPAC(1) receptor subtype which shares similar high affinity for VIP and PACAP-27. 2. The stoichiometric binding parameters characterizing the (125)I-VIP and (125)I-PACAP-27 binding to these receptors were determined. 3. We found that VIP (EC(50) approximately 7.6 nM) and PACAP-27 (EC(50) approximately 10 nM) stimulated glibenclamide-sensitive and DIDS-insensitive iodide efflux in Calu-3 cells. 4. The protein kinase A (PKA) inhibitor, H-89 and the protein kinase C (PKC) inhibitor, chelerythrine chloride prevented activation by both peptides demonstrating that PKA and PKC are part of the signaling pathway. This profile corresponds to the pharmacological signature of CFTR. 5. In the cystic fibrosis airway epithelial IB3-1 cell lacking functional CFTR but expressing VPAC(1) receptors, neither VIP, PACAP-27 nor forskolin stimulated chloride transport. 6. Ussing chamber experiments demonstrated stimulation of CFTR-dependent short-circuit currents by VIP or PACAP-27 applied to the basolateral but not to the apical side of Calu-3 cells monolayers. 7. This study shows the stimulation in human bronchial epithelial cells of CFTR-dependent chloride secretion following activation by VIP and PACAP-27 of basolateral VPAC(1) receptors." @default.
• W1991799302 created "2016-06-24" @default.
• W1991799302 creator A5004728898 @default.
• W1991799302 creator A5006831708 @default.
• W1991799302 creator A5010602401 @default.
• W1991799302 creator A5012699429 @default.
• W1991799302 creator A5020239167 @default.
• W1991799302 creator A5053935194 @default.
• W1991799302 creator A5075040595 @default.
• W1991799302 date "2004-02-01" @default.
• W1991799302 modified "2023-10-14" @default.
• W1991799302 title "Activation of VPAC₁receptors by VIP and PACAP-27 in human bronchial epithelial cells induces CFTR-dependent chloride secretion" @default.
• W1991799302 cites W1593074195 @default.
• W1991799302 cites W1799683217 @default.
• W1991799302 cites W1819465909 @default.
• W1991799302 cites W1897443070 @default.
• W1991799302 cites W1918494965 @default.
• W1991799302 cites W1983114544 @default.
• W1991799302 cites W1984447869 @default.
• W1991799302 cites W1993913617 @default.
• W1991799302 cites W1995335896 @default.
• W1991799302 cites W1996709623 @default.
• W1991799302 cites W1999662264 @default.
• W1991799302 cites W2004612241 @default.
• W1991799302 cites W2006346685 @default.
• W1991799302 cites W2014035772 @default.
• W1991799302 cites W2014870167 @default.
• W1991799302 cites W2017632365 @default.
• W1991799302 cites W2020212141 @default.
• W1991799302 cites W2023901212 @default.
• W1991799302 cites W2037501344 @default.
• W1991799302 cites W2037622315 @default.
• W1991799302 cites W2039599894 @default.
• W1991799302 cites W2042843548 @default.
• W1991799302 cites W2045419222 @default.
• W1991799302 cites W2046470493 @default.
• W1991799302 cites W2049914201 @default.
• W1991799302 cites W2053672287 @default.
• W1991799302 cites W2064006285 @default.
• W1991799302 cites W2069562921 @default.
• W1991799302 cites W2076091117 @default.
• W1991799302 cites W2079659063 @default.
• W1991799302 cites W2081048298 @default.
• W1991799302 cites W2095000642 @default.
• W1991799302 cites W2098498572 @default.
• W1991799302 cites W2135339549 @default.
• W1991799302 cites W2137346267 @default.
• W1991799302 cites W2150447279 @default.
• W1991799302 cites W2160894539 @default.
• W1991799302 cites W2188667633 @default.
• W1991799302 cites W2257252244 @default.
• W1991799302 cites W2275892949 @default.
• W1991799302 cites W2320090661 @default.
• W1991799302 cites W2335805947 @default.
• W1991799302 cites W2341580088 @default.
• W1991799302 cites W2342271276 @default.
• W1991799302 cites W2409976770 @default.
• W1991799302 cites W2415909160 @default.
• W1991799302 cites W2470078903 @default.
• W1991799302 cites W2777476097 @default.
• W1991799302 cites W4236558664 @default.
• W1991799302 cites W4285719527 @default.
• W1991799302 doi "https://doi.org/10.1038/sj.bjp.0705597" @default.
• W1991799302 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1574226" @default.
• W1991799302 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14744818" @default.
• W1991799302 hasPublicationYear "2004" @default.
• W1991799302 type Work @default.
• W1991799302 sameAs 1991799302 @default.
• W1991799302 citedByCount "49" @default.
• W1991799302 countsByYear W19917993022012 @default.
• W1991799302 countsByYear W19917993022013 @default.
• W1991799302 countsByYear W19917993022016 @default.
• W1991799302 countsByYear W19917993022018 @default.
• W1991799302 countsByYear W19917993022019 @default.
• W1991799302 countsByYear W19917993022020 @default.
• W1991799302 countsByYear W19917993022021 @default.
• W1991799302 countsByYear W19917993022022 @default.
• W1991799302 crossrefType "journal-article" @default.
• W1991799302 hasAuthorship W1991799302A5004728898 @default.
• W1991799302 hasAuthorship W1991799302A5006831708 @default.
• W1991799302 hasAuthorship W1991799302A5010602401 @default.
• W1991799302 hasAuthorship W1991799302A5012699429 @default.
• W1991799302 hasAuthorship W1991799302A5020239167 @default.
• W1991799302 hasAuthorship W1991799302A5053935194 @default.
• W1991799302 hasAuthorship W1991799302A5075040595 @default.
• W1991799302 hasBestOaLocation W19917993021 @default.
• W1991799302 hasConcept C118303440 @default.
• W1991799302 hasConcept C126322002 @default.
• W1991799302 hasConcept C134018914 @default.
• W1991799302 hasConcept C145822097 @default.
• W1991799302 hasConcept C170493617 @default.
• W1991799302 hasConcept C185592680 @default.
• W1991799302 hasConcept C195794163 @default.
• W1991799302 hasConcept C24998067 @default.
• W1991799302 hasConcept C2776108454 @default.
• W1991799302 hasConcept C2776938444 @default.
• W1991799302 hasConcept C2778428886 @default.
• W1991799302 hasConcept C2779276759 @default.

• next