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- W1991820295 abstract "Phosphorylation is a major post-translational mechanism of regulation that frequently targets disordered protein domains, but it remains unclear how phosphorylation modulates disordered states of proteins. Here we determine the kinetics and energetics of a disordered protein domain the kinase-inducible domain (KID) of the transcription factor CREB and that of its phosphorylated form pKID, using high-throughput molecular dynamic simulations. We identify the presence of a metastable, partially ordered state with a 60-fold slowdown in conformational kinetics that arises due to phosphorylation, kinetically stabilizing residues known to participate in an early binding intermediate. We show that this effect is only partially reconstituted by mutation to glutamate, indicating that the phosphate is uniquely required for the long-lived state to arise. This mechanism of kinetic modulation could be important for regulation beyond conformational equilibrium shifts. Protein phosphorylation is a frequent biological occurrence, but the way in which this process modulates the disordered states of proteins is poorly understood. Here, the authors present simulations of the effect of phosphorylation on the kinetics and energetics of a disordered protein." @default.
- W1991820295 created "2016-06-24" @default.
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- W1991820295 date "2014-10-28" @default.
- W1991820295 modified "2023-09-25" @default.
- W1991820295 title "Kinetic modulation of a disordered protein domain by phosphorylation" @default.
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- W1991820295 doi "https://doi.org/10.1038/ncomms6272" @default.
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