Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991828699> ?p ?o ?g. }
- W1991828699 abstract "1. Apical membrane K+ channels in turtle colon were identified and characterized using current fluctuation analysis. 2. Under short-circuit conditions in NaCl-Ringer solution, the power density spectrum (PDS) of the short-circuit current (Isc) sometimes exhibited a clearly discernible Lorentzian component, indicating spontaneous fluctuations produced by a population of apical ion channels. The Lorentzian component had a characteristic corner frequency (fc) which averaged 10.2 +/- 0.9 Hz (mean +/- S.E.M., n = 20). 3. The power of the spontaneous fluctuations was enhanced (So increased) by manoeuvres that depolarize the apical membrane electrical potential (Va). Discernible fluctuations were enhanced or induced by raising the serosal K+ concentration ([K+]s = 50-115 mM, Na+ replacement), by clamping the transepithelial potential (Vt) to serosa-positive values, or by blocking basolateral K+ channels with Ba2+. 4. Mucosal amiloride (100 microM) attenuated the spontaneous fluctuations observed in NaCl-Ringer solution but had no effect in the presence of serosal high K+, indicating that amiloride did not block the K(+)-permeable channels but these channels resided in the same cells as the amiloride-sensitive Na+ channels. 5. Raising the mucosal K+ concentration attenuated spontaneous fluctuations. 6. In the presence of serosal high K+ and mucosal amiloride, the spontaneous fluctuations were often accompanied by a reversed Isc consistent with K+ secretion. These conditions were used to test the effects of putative channel blockers. 7. Mucosal Ba2+ and tetraethylammonium (TEA+) were effective inhibitors of the spontaneous fluctuations and the reversed Isc. At a concentration of 10 mM, TEA+ was maximally effective but the TEA+ analogues tetramethylammonium (TMA+) and tetrapropylammonium (TPrA+) were much less effective. Mucosal Rb+ or Cs+ did not inhibit at a concentration of 10 mM. 8. Mucosal lidocaine (200 microM), quinidine (200 microM), or diphenylamine-2-carboxylate (DPC, 1 mM) had little or no effect on the spontaneous fluctuations and reversed Isc. Quinine (100 microM), 4-aminopyridine (1 mM), and apamin (100 nM) were also without effect. 9. Mucosal TEA+ (10 mM) abolished the active secretory K+ flux measured in the presence of serosa-positive transepithelial potentials. 10. These experiments identified a population of TEA(+)-sensitive, apical K+ channels which mediate active K+ secretion in turtle colon. Sensitivity to external TEA+ distinguishes these channels from basolateral K+ channels in turtle colon and demonstrates similarity to apical K+ channels in mammalian colon." @default.
- W1991828699 created "2016-06-24" @default.
- W1991828699 creator A5008362701 @default.
- W1991828699 creator A5023719756 @default.
- W1991828699 creator A5042528952 @default.
- W1991828699 date "1993-03-01" @default.
- W1991828699 modified "2023-09-24" @default.
- W1991828699 title "Tetraethylammonium-sensitive apical K+ channels mediating K+ secretion by turtle colon." @default.
- W1991828699 cites W1569014539 @default.
- W1991828699 cites W157041128 @default.
- W1991828699 cites W1909114096 @default.
- W1991828699 cites W1972631430 @default.
- W1991828699 cites W1997102479 @default.
- W1991828699 cites W1999795496 @default.
- W1991828699 cites W2009035521 @default.
- W1991828699 cites W2010455387 @default.
- W1991828699 cites W2025884619 @default.
- W1991828699 cites W2031864235 @default.
- W1991828699 cites W2040801479 @default.
- W1991828699 cites W2049815711 @default.
- W1991828699 cites W2052465981 @default.
- W1991828699 cites W2074948539 @default.
- W1991828699 cites W2076610634 @default.
- W1991828699 cites W2079862434 @default.
- W1991828699 cites W2089609865 @default.
- W1991828699 cites W2092469506 @default.
- W1991828699 cites W2103760667 @default.
- W1991828699 cites W2117245601 @default.
- W1991828699 cites W2125210021 @default.
- W1991828699 cites W2185701537 @default.
- W1991828699 cites W2229407446 @default.
- W1991828699 cites W2243541242 @default.
- W1991828699 cites W2280264547 @default.
- W1991828699 cites W2313272858 @default.
- W1991828699 cites W2412210093 @default.
- W1991828699 cites W2412217596 @default.
- W1991828699 cites W2417296939 @default.
- W1991828699 cites W2467945678 @default.
- W1991828699 cites W257281402 @default.
- W1991828699 cites W2920633646 @default.
- W1991828699 cites W3164257535 @default.
- W1991828699 doi "https://doi.org/10.1113/jphysiol.1993.sp019577" @default.
- W1991828699 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1175323" @default.
- W1991828699 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8392578" @default.
- W1991828699 hasPublicationYear "1993" @default.
- W1991828699 type Work @default.
- W1991828699 sameAs 1991828699 @default.
- W1991828699 citedByCount "7" @default.
- W1991828699 countsByYear W19918286992020 @default.
- W1991828699 crossrefType "journal-article" @default.
- W1991828699 hasAuthorship W1991828699A5008362701 @default.
- W1991828699 hasAuthorship W1991828699A5023719756 @default.
- W1991828699 hasAuthorship W1991828699A5042528952 @default.
- W1991828699 hasBestOaLocation W19918286992 @default.
- W1991828699 hasConcept C12554922 @default.
- W1991828699 hasConcept C178790620 @default.
- W1991828699 hasConcept C181911157 @default.
- W1991828699 hasConcept C185592680 @default.
- W1991828699 hasConcept C2779608910 @default.
- W1991828699 hasConcept C2780918503 @default.
- W1991828699 hasConcept C2781295685 @default.
- W1991828699 hasConcept C3018436504 @default.
- W1991828699 hasConcept C4141045 @default.
- W1991828699 hasConcept C41625074 @default.
- W1991828699 hasConcept C517785266 @default.
- W1991828699 hasConcept C537181965 @default.
- W1991828699 hasConcept C55493867 @default.
- W1991828699 hasConcept C86803240 @default.
- W1991828699 hasConceptScore W1991828699C12554922 @default.
- W1991828699 hasConceptScore W1991828699C178790620 @default.
- W1991828699 hasConceptScore W1991828699C181911157 @default.
- W1991828699 hasConceptScore W1991828699C185592680 @default.
- W1991828699 hasConceptScore W1991828699C2779608910 @default.
- W1991828699 hasConceptScore W1991828699C2780918503 @default.
- W1991828699 hasConceptScore W1991828699C2781295685 @default.
- W1991828699 hasConceptScore W1991828699C3018436504 @default.
- W1991828699 hasConceptScore W1991828699C4141045 @default.
- W1991828699 hasConceptScore W1991828699C41625074 @default.
- W1991828699 hasConceptScore W1991828699C517785266 @default.
- W1991828699 hasConceptScore W1991828699C537181965 @default.
- W1991828699 hasConceptScore W1991828699C55493867 @default.
- W1991828699 hasConceptScore W1991828699C86803240 @default.
- W1991828699 hasLocation W19918286991 @default.
- W1991828699 hasLocation W19918286992 @default.
- W1991828699 hasLocation W19918286993 @default.
- W1991828699 hasLocation W19918286994 @default.
- W1991828699 hasOpenAccess W1991828699 @default.
- W1991828699 hasPrimaryLocation W19918286991 @default.
- W1991828699 hasRelatedWork W1991828699 @default.
- W1991828699 hasRelatedWork W2003658133 @default.
- W1991828699 hasRelatedWork W2012605036 @default.
- W1991828699 hasRelatedWork W2022788677 @default.
- W1991828699 hasRelatedWork W2070293291 @default.
- W1991828699 hasRelatedWork W2087015066 @default.
- W1991828699 hasRelatedWork W2138728898 @default.
- W1991828699 hasRelatedWork W2337748810 @default.
- W1991828699 hasRelatedWork W2396652815 @default.
- W1991828699 hasRelatedWork W2509395642 @default.
- W1991828699 isParatext "false" @default.
- W1991828699 isRetracted "false" @default.