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- W1991862270 abstract "The syntheses of the four novel, base protected 5′-(S-triphenylmethyl)mercapto-2′,5′-dideoxyribonucleoside-3′-0-(2-cyanoethyl N,N-diisopropylphosphoramidites) are described. These compounds have been used to prepare 5′-(S-triphenylmethyl) mercapto-oligodeoxyribonucleotides, which are readily purified by reversed phase h.p.l.c, owing to the highly lipophilic trityl group. After cleavage of the S-trityl group by silver or mercuric ions, the free thiol moiety can be coupled to a wide variety of reagents, generating very useful probes. Fluorescent labelled 5′-mercapto-oligodeoxyribo-nucleotides are being used for automated DNA sequencing without radioactivity, and heavy metal labelled 5′-mercapto-oligonucleotides will be used in X-ray crystallography." @default.
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- W1991862270 date "1987-01-01" @default.
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- W1991862270 title "The synthesis of protected 5′-mercapto-2′,5′-dideoxyribonucleoside-3′-0-phosphorainidites; uses of 5′-mercapto-oligodeoxyribonucleotides" @default.
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- W1991862270 doi "https://doi.org/10.1093/nar/15.12.4837" @default.
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