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- W1991863522 abstract "The aim of this experiment was to study the effect of the most frequently used preservation media for soil invertebrates on the 15N concentration in Folsomia candida (Collembola) during extraction and storage. Two stocks of F. candida were fed with food containing either a low or a high quantity of 15N for at least 2 months. Specimens from both stocks were stored in different preservatives for 1, 5 and 27 weeks. Specimens were preserved in eleven different solutions during the first week: 70% ethyl alcohol, 70% ethyl alcohol with 5% glycerin, 1% picric acid, distilled water, tap water, 70% isopropanol, 0.5% salicylic acid, saturated NaCl, 0.5% sodium benzoate, 25% ethyl glycol, and 10% formalin. Some specimens were killed immediately using heat (60°C). Another group of animals was kept frozen at −18°C. The specimens for the 5- and 27-week experiments were kept in 70% ethyl alcohol for the first week and subsequently one group was transferred into 90% ethyl alcohol, a second into 4% formalin and a third group was frozen. The results show that the different preservatives influenced the 15N concentration. The alcohols did not change the 15N concentration while picric acid and sodium benzoate led to reduced amounts of the isotope. The other liquids had modest effects. Freezing or preservation in ethyl alcohol or formalin proved to be reliable methods of storage for the purpose of a 15N analysis." @default.
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- W1991863522 date "1998-09-01" @default.
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- W1991863522 title "The effects of different methods of preservation on the 15N concentration in Folsomia candida (Collembola)" @default.
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- W1991863522 doi "https://doi.org/10.1016/s0929-1393(98)00061-4" @default.
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