FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991895890> ?p ?o ?g. }

• W1991895890 endingPage "236" @default.
• W1991895890 startingPage "224" @default.
• W1991895890 abstract "This study shows that there is only a negligible difference in actomyosin function in the in vitro motility assay among actin filaments labeled with Rhodamine phalloidin (RhPh), Alexa-488 phalloidin (APh), and biotin-XX phalloidin (BPh). Similar results were obtained at varying ionic strengths (0.02–0.13 M), in the presence of imidazole or 3-[N-morpholino]propanesulfonic acid (MOPS) buffer, and at varying MgATP concentrations (0.1–3 mM). If RhPh- and APh-labeled filaments were studied in a given flow cell, there was minimal variability in sliding velocity between the fluorophores (standard deviation of 3% of the absolute sliding velocity). The variability was considerably smaller than that between flow cells, allowing us to use dual labeling of different actin types and then apply analysis of variance to detect minor functional differences between them. Using this method, we could statistically verify a 4% difference (P < 0.001) in sliding velocity (3 mM Mg ATP) between cardiac and skeletal muscle actin. Suggested improvements of the method would readily allow the detection of even smaller differences. We discuss implications of the results for nanotechnological applications, understanding actomyosin function, and reducing experimental costs and the use of laboratory animals." @default.
• W1991895890 created "2016-06-24" @default.
• W1991895890 creator A5003694914 @default.
• W1991895890 creator A5003988560 @default.
• W1991895890 date "2005-03-01" @default.
• W1991895890 modified "2023-10-10" @default.
• W1991895890 title "Detection of small differences in actomyosin function using actin labeled with different phalloidin conjugates" @default.
• W1991895890 cites W1496654933 @default.
• W1991895890 cites W1501204944 @default.
• W1991895890 cites W1889656622 @default.
• W1991895890 cites W1965683001 @default.
• W1991895890 cites W1975714515 @default.
• W1991895890 cites W1977522933 @default.
• W1991895890 cites W1980941446 @default.
• W1991895890 cites W1982752211 @default.
• W1991895890 cites W199045229 @default.
• W1991895890 cites W1997831845 @default.
• W1991895890 cites W2002732971 @default.
• W1991895890 cites W2022001508 @default.
• W1991895890 cites W2026836008 @default.
• W1991895890 cites W2028207310 @default.
• W1991895890 cites W2036534655 @default.
• W1991895890 cites W2052976658 @default.
• W1991895890 cites W2059529011 @default.
• W1991895890 cites W2063130268 @default.
• W1991895890 cites W206708297 @default.
• W1991895890 cites W2067386885 @default.
• W1991895890 cites W2089114409 @default.
• W1991895890 cites W2090251835 @default.
• W1991895890 cites W2110720259 @default.
• W1991895890 cites W2117935280 @default.
• W1991895890 cites W2118905867 @default.
• W1991895890 cites W2143464032 @default.
• W1991895890 cites W2145780151 @default.
• W1991895890 cites W2149295320 @default.
• W1991895890 cites W2151808726 @default.
• W1991895890 cites W217559604 @default.
• W1991895890 cites W2288293234 @default.
• W1991895890 cites W308629480 @default.
• W1991895890 cites W789458867 @default.
• W1991895890 cites W952792670 @default.
• W1991895890 doi "https://doi.org/10.1016/j.ab.2004.12.012" @default.
• W1991895890 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15745742" @default.
• W1991895890 hasPublicationYear "2005" @default.
• W1991895890 type Work @default.
• W1991895890 sameAs 1991895890 @default.
• W1991895890 citedByCount "29" @default.
• W1991895890 countsByYear W19918958902012 @default.
• W1991895890 countsByYear W19918958902013 @default.
• W1991895890 countsByYear W19918958902016 @default.
• W1991895890 countsByYear W19918958902017 @default.
• W1991895890 countsByYear W19918958902018 @default.
• W1991895890 countsByYear W19918958902021 @default.
• W1991895890 countsByYear W19918958902022 @default.
• W1991895890 countsByYear W19918958902023 @default.
• W1991895890 crossrefType "journal-article" @default.
• W1991895890 hasAuthorship W1991895890A5003694914 @default.
• W1991895890 hasAuthorship W1991895890A5003988560 @default.
• W1991895890 hasConcept C120665830 @default.
• W1991895890 hasConcept C121332964 @default.
• W1991895890 hasConcept C12554922 @default.
• W1991895890 hasConcept C125705527 @default.
• W1991895890 hasConcept C142669718 @default.
• W1991895890 hasConcept C1491633281 @default.
• W1991895890 hasConcept C185592680 @default.
• W1991895890 hasConcept C2776861759 @default.
• W1991895890 hasConcept C2779916640 @default.
• W1991895890 hasConcept C55493867 @default.
• W1991895890 hasConcept C58207958 @default.
• W1991895890 hasConcept C86803240 @default.
• W1991895890 hasConcept C91881484 @default.
• W1991895890 hasConcept C95444343 @default.
• W1991895890 hasConceptScore W1991895890C120665830 @default.
• W1991895890 hasConceptScore W1991895890C121332964 @default.
• W1991895890 hasConceptScore W1991895890C12554922 @default.
• W1991895890 hasConceptScore W1991895890C125705527 @default.
• W1991895890 hasConceptScore W1991895890C142669718 @default.
• W1991895890 hasConceptScore W1991895890C1491633281 @default.
• W1991895890 hasConceptScore W1991895890C185592680 @default.
• W1991895890 hasConceptScore W1991895890C2776861759 @default.
• W1991895890 hasConceptScore W1991895890C2779916640 @default.
• W1991895890 hasConceptScore W1991895890C55493867 @default.
• W1991895890 hasConceptScore W1991895890C58207958 @default.
• W1991895890 hasConceptScore W1991895890C86803240 @default.
• W1991895890 hasConceptScore W1991895890C91881484 @default.
• W1991895890 hasConceptScore W1991895890C95444343 @default.
• W1991895890 hasIssue "2" @default.
• W1991895890 hasLocation W19918958901 @default.
• W1991895890 hasLocation W19918958902 @default.
• W1991895890 hasOpenAccess W1991895890 @default.
• W1991895890 hasPrimaryLocation W19918958901 @default.
• W1991895890 hasRelatedWork W1560179018 @default.
• W1991895890 hasRelatedWork W1965784776 @default.
• W1991895890 hasRelatedWork W1986466301 @default.
• W1991895890 hasRelatedWork W2084822214 @default.
• W1991895890 hasRelatedWork W2121901256 @default.
• W1991895890 hasRelatedWork W2320626004 @default.
• W1991895890 hasRelatedWork W2397751340 @default.

• next