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- W1991898103 abstract "Direct electron transfer of horseradish peroxidase (HRP) immobilized in graphene oxide (GO)–Nafion nanocomposite film and its application as a new biosensor was investigated with electrochemical methods. Immobilized HRP shows a pair of well-defined redox waves and retains its bioelectrocatalytic activity for the reduction of H2O2 and O2. As a new sensor with excellent electrocatalytic response to the reduction of H2O2 and O2, calibrations with good linear relationships were obtained from 1.0 μmol L−1 to 1.0 mmol L−1 for H2O2 and from 0.5 μmol L−1 to 18.6 μmol L−1 for O2 with the detection limits of 4.0 × 10−7 mol L−1 for H2O2 and 1.0 × 10−7 mol L−1 for O2 at a signal-to-noise ratio of 3. Additionally, the responses showed Michaelis–Menten behavior with Kmapp values of 0.684 mmol L−1 for H2O2 and 0.0160 mmol L−1 for O2. Moreover, the cathodic peak current of an HRP/GO/Nafion/GCE biosensor decreases by less than 5% after 4 weeks. These results reveal that GO can be conveniently incorporated into a polymer nanocomposite for fabrication of new GO-based biosensors." @default.
- W1991898103 created "2016-06-24" @default.
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- W1991898103 date "2012-03-01" @default.
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- W1991898103 title "Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in graphene oxide–Nafion nanocomposite film" @default.
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- W1991898103 doi "https://doi.org/10.1016/j.electacta.2012.01.029" @default.
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