Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991921608> ?p ?o ?g. }
- W1991921608 endingPage "70" @default.
- W1991921608 startingPage "60" @default.
- W1991921608 abstract "Cell-therapy was proposed to be a promising tool in case of death or impairment of specific cell types. Correct identification of implanted cells became crucial when evaluating the success of transplantation therapy. Various methods of cell labeling have been employed in previously published studies. The use of intrinsic signaling of green fluorescent protein (GFP) has led to a well known controversy in the field of cardiovascular research. We encountered similar methodological pitfalls after transplantation of GFP-transfected embryonic stem cells into rat brains following traumatic brain injury (TBI). As the identification of implanted graft by intrinsic autofluorescence failed, anti-GFP labeling coupled to fluorescent and conventional antibodies was needed to visualize the implanted cells. Furthermore, different cell types with strong intrinsic autofluorescence were found at the sites of injury and transplantation, thus mimicking the implanted stem cells. GFP-positive stem cells were correctly localized, using advanced histological techniques. The activation of microglia/macrophages, accompanying the transplantation post TBI, was shown to be a significant source of artefacts, interfering with correct identification of implanted stem cells. Dependent on the strategy of stem cell tracking, the phagocytosis of implanted cells as observed in this study, might also impede the interpretation of results. Critical appraisal of previously published data as well as a review of different histological techniques provide tools for a more accurate identification of transplanted stem cells." @default.
- W1991921608 created "2016-06-24" @default.
- W1991921608 creator A5001580510 @default.
- W1991921608 creator A5018453143 @default.
- W1991921608 creator A5018636174 @default.
- W1991921608 creator A5018681115 @default.
- W1991921608 creator A5051949436 @default.
- W1991921608 creator A5058644048 @default.
- W1991921608 creator A5070654690 @default.
- W1991921608 creator A5081061243 @default.
- W1991921608 creator A5083746732 @default.
- W1991921608 creator A5084405911 @default.
- W1991921608 date "2013-04-01" @default.
- W1991921608 modified "2023-10-14" @default.
- W1991921608 title "Pitfalls and fallacies interfering with correct identification of embryonic stem cells implanted into the brain after experimental traumatic injury" @default.
- W1991921608 cites W1631250427 @default.
- W1991921608 cites W174854816 @default.
- W1991921608 cites W1965245166 @default.
- W1991921608 cites W1965449838 @default.
- W1991921608 cites W1968465823 @default.
- W1991921608 cites W1970137308 @default.
- W1991921608 cites W1972582749 @default.
- W1991921608 cites W1976102520 @default.
- W1991921608 cites W1976551244 @default.
- W1991921608 cites W1978286697 @default.
- W1991921608 cites W1982228967 @default.
- W1991921608 cites W1983939417 @default.
- W1991921608 cites W1984323174 @default.
- W1991921608 cites W1985269282 @default.
- W1991921608 cites W1985823190 @default.
- W1991921608 cites W1986415053 @default.
- W1991921608 cites W1989417097 @default.
- W1991921608 cites W1989744404 @default.
- W1991921608 cites W1991374176 @default.
- W1991921608 cites W1991608077 @default.
- W1991921608 cites W1994410316 @default.
- W1991921608 cites W1994499810 @default.
- W1991921608 cites W1995869509 @default.
- W1991921608 cites W1999348847 @default.
- W1991921608 cites W1999711808 @default.
- W1991921608 cites W1999934730 @default.
- W1991921608 cites W2002038047 @default.
- W1991921608 cites W2003179114 @default.
- W1991921608 cites W2004055904 @default.
- W1991921608 cites W2005910466 @default.
- W1991921608 cites W2016823790 @default.
- W1991921608 cites W2018708343 @default.
- W1991921608 cites W2038285174 @default.
- W1991921608 cites W2039596366 @default.
- W1991921608 cites W2043206878 @default.
- W1991921608 cites W2051600991 @default.
- W1991921608 cites W2051804874 @default.
- W1991921608 cites W2058027038 @default.
- W1991921608 cites W2058813328 @default.
- W1991921608 cites W2061976748 @default.
- W1991921608 cites W2066140090 @default.
- W1991921608 cites W2068380542 @default.
- W1991921608 cites W2070589883 @default.
- W1991921608 cites W2073294682 @default.
- W1991921608 cites W2076194369 @default.
- W1991921608 cites W2080950947 @default.
- W1991921608 cites W2082678885 @default.
- W1991921608 cites W2082752553 @default.
- W1991921608 cites W2084671887 @default.
- W1991921608 cites W2085874686 @default.
- W1991921608 cites W2087200027 @default.
- W1991921608 cites W2089572671 @default.
- W1991921608 cites W2090041902 @default.
- W1991921608 cites W2097900305 @default.
- W1991921608 cites W2103274786 @default.
- W1991921608 cites W2109794889 @default.
- W1991921608 cites W2118437843 @default.
- W1991921608 cites W2125155374 @default.
- W1991921608 cites W2129006023 @default.
- W1991921608 cites W2142089995 @default.
- W1991921608 cites W2150398616 @default.
- W1991921608 cites W2150727529 @default.
- W1991921608 cites W2152845218 @default.
- W1991921608 cites W2159982756 @default.
- W1991921608 cites W2168341415 @default.
- W1991921608 cites W2169254515 @default.
- W1991921608 cites W2314333161 @default.
- W1991921608 cites W4301205864 @default.
- W1991921608 doi "https://doi.org/10.1016/j.jneumeth.2013.02.012" @default.
- W1991921608 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23454685" @default.
- W1991921608 hasPublicationYear "2013" @default.
- W1991921608 type Work @default.
- W1991921608 sameAs 1991921608 @default.
- W1991921608 citedByCount "15" @default.
- W1991921608 countsByYear W19919216082013 @default.
- W1991921608 countsByYear W19919216082014 @default.
- W1991921608 countsByYear W19919216082015 @default.
- W1991921608 countsByYear W19919216082016 @default.
- W1991921608 countsByYear W19919216082017 @default.
- W1991921608 countsByYear W19919216082020 @default.
- W1991921608 countsByYear W19919216082021 @default.
- W1991921608 countsByYear W19919216082022 @default.
- W1991921608 countsByYear W19919216082023 @default.