Matches in SemOpenAlex for { <https://semopenalex.org/work/W1991964554> ?p ?o ?g. }
- W1991964554 endingPage "e19970" @default.
- W1991964554 startingPage "e19970" @default.
- W1991964554 abstract "Chronic inflammation of the lung, as a consequence of persistent bacterial infections by several opportunistic pathogens represents the main cause of mortality and morbidity in cystic fibrosis (CF) patients. Mechanisms leading to increased susceptibility to bacterial infections in CF are not completely known, although the involvement of cystic fibrosis transmembrane conductance regulator (CFTR) in microbicidal functions of macrophages is emerging. Tissue macrophages differentiate in situ from infiltrating monocytes, additionally, mature macrophages from different tissues, although having a number of common activities, exhibit variation in some molecular and cellular functions. In order to highlight possible intrinsic macrophage defects due to CFTR dysfunction, we have focused our attention on in vitro differentiated macrophages from human peripheral blood monocytes. Here we report on the contribution of CFTR in the bactericidal activity against Pseudomonas aeruginosa of monocyte derived human macrophages. At first, by real time PCR, immunofluorescence and patch clamp recordings we demonstrated that CFTR is expressed and is mainly localized to surface plasma membranes of human monocyte derived macrophages (MDM) where it acts as a cAMP-dependent chloride channel. Next, we evaluated the bactericidal activity of P. aeruginosa infected macrophages from healthy donors and CF patients by antibiotic protection assays. Our results demonstrate that control and CF macrophages do not differ in the phagocytic activity when infected with P. aeruginosa. Rather, although a reduction of intracellular live bacteria was detected in both non-CF and CF cells, the percentage of surviving bacteria was significantly higher in CF cells. These findings further support the role of CFTR in the fundamental functions of innate immune cells including eradication of bacterial infections by macrophages." @default.
- W1991964554 created "2016-06-24" @default.
- W1991964554 creator A5012948165 @default.
- W1991964554 creator A5028416545 @default.
- W1991964554 creator A5036082730 @default.
- W1991964554 creator A5046064422 @default.
- W1991964554 creator A5063922011 @default.
- W1991964554 creator A5067107046 @default.
- W1991964554 creator A5068820892 @default.
- W1991964554 creator A5072178306 @default.
- W1991964554 creator A5078833835 @default.
- W1991964554 creator A5089032781 @default.
- W1991964554 creator A5089270717 @default.
- W1991964554 date "2011-05-18" @default.
- W1991964554 modified "2023-09-27" @default.
- W1991964554 title "Dysfunctional CFTR Alters the Bactericidal Activity of Human Macrophages against Pseudomonas aeruginosa" @default.
- W1991964554 cites W1509065673 @default.
- W1991964554 cites W1974796951 @default.
- W1991964554 cites W1982956224 @default.
- W1991964554 cites W1985931909 @default.
- W1991964554 cites W1988243490 @default.
- W1991964554 cites W1996302426 @default.
- W1991964554 cites W2009667219 @default.
- W1991964554 cites W2023339317 @default.
- W1991964554 cites W2024688910 @default.
- W1991964554 cites W2039599894 @default.
- W1991964554 cites W2045758625 @default.
- W1991964554 cites W2062906311 @default.
- W1991964554 cites W2064434946 @default.
- W1991964554 cites W2069717071 @default.
- W1991964554 cites W2090605523 @default.
- W1991964554 cites W2091561409 @default.
- W1991964554 cites W2095966606 @default.
- W1991964554 cites W2096684565 @default.
- W1991964554 cites W2100619015 @default.
- W1991964554 cites W2111797194 @default.
- W1991964554 cites W2125391335 @default.
- W1991964554 cites W2125428336 @default.
- W1991964554 cites W2125762027 @default.
- W1991964554 cites W2128876424 @default.
- W1991964554 cites W2137149853 @default.
- W1991964554 cites W2141098072 @default.
- W1991964554 cites W2143695671 @default.
- W1991964554 cites W2158024739 @default.
- W1991964554 cites W2164652755 @default.
- W1991964554 cites W2166716399 @default.
- W1991964554 cites W2168469435 @default.
- W1991964554 cites W2615630650 @default.
- W1991964554 cites W4233502188 @default.
- W1991964554 doi "https://doi.org/10.1371/journal.pone.0019970" @default.
- W1991964554 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3097223" @default.
- W1991964554 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21625641" @default.
- W1991964554 hasPublicationYear "2011" @default.
- W1991964554 type Work @default.
- W1991964554 sameAs 1991964554 @default.
- W1991964554 citedByCount "123" @default.
- W1991964554 countsByYear W19919645542012 @default.
- W1991964554 countsByYear W19919645542013 @default.
- W1991964554 countsByYear W19919645542014 @default.
- W1991964554 countsByYear W19919645542015 @default.
- W1991964554 countsByYear W19919645542016 @default.
- W1991964554 countsByYear W19919645542017 @default.
- W1991964554 countsByYear W19919645542018 @default.
- W1991964554 countsByYear W19919645542019 @default.
- W1991964554 countsByYear W19919645542020 @default.
- W1991964554 countsByYear W19919645542021 @default.
- W1991964554 countsByYear W19919645542022 @default.
- W1991964554 countsByYear W19919645542023 @default.
- W1991964554 crossrefType "journal-article" @default.
- W1991964554 hasAuthorship W1991964554A5012948165 @default.
- W1991964554 hasAuthorship W1991964554A5028416545 @default.
- W1991964554 hasAuthorship W1991964554A5036082730 @default.
- W1991964554 hasAuthorship W1991964554A5046064422 @default.
- W1991964554 hasAuthorship W1991964554A5063922011 @default.
- W1991964554 hasAuthorship W1991964554A5067107046 @default.
- W1991964554 hasAuthorship W1991964554A5068820892 @default.
- W1991964554 hasAuthorship W1991964554A5072178306 @default.
- W1991964554 hasAuthorship W1991964554A5078833835 @default.
- W1991964554 hasAuthorship W1991964554A5089032781 @default.
- W1991964554 hasAuthorship W1991964554A5089270717 @default.
- W1991964554 hasBestOaLocation W19919645541 @default.
- W1991964554 hasConcept C153726182 @default.
- W1991964554 hasConcept C202751555 @default.
- W1991964554 hasConcept C203014093 @default.
- W1991964554 hasConcept C2776914184 @default.
- W1991964554 hasConcept C2776938444 @default.
- W1991964554 hasConcept C2777637488 @default.
- W1991964554 hasConcept C2778428886 @default.
- W1991964554 hasConcept C2779244956 @default.
- W1991964554 hasConcept C2781184567 @default.
- W1991964554 hasConcept C28406088 @default.
- W1991964554 hasConcept C523546767 @default.
- W1991964554 hasConcept C54355233 @default.
- W1991964554 hasConcept C55493867 @default.
- W1991964554 hasConcept C79879829 @default.
- W1991964554 hasConcept C86803240 @default.
- W1991964554 hasConcept C89423630 @default.
- W1991964554 hasConcept C95444343 @default.