Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992004187> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1992004187 endingPage "569a" @default.
- W1992004187 startingPage "569a" @default.
- W1992004187 abstract "Myosin VI is an unconventional myosin motor that moves towards the minus end of actin filaments. The motor is known to interact with various binding partners and function as an anchor and a vesicle carrier in many diverse functions, including endocytosis and exocytosis. The multiple functions are likely to be regulated through the binding partners. The functional units of the motor (monomers/dimers) and the effects of binding partners remain unclear. Here we present a quantitative FRET based assay to measure the association of binding partners and determine the effect upon the oligomeric state of myosin VI. We have shown that myosin VI tail forms relatively tight interactions with the binding partners NDP52 (Kd 1 μM) and Dab2 (Kd 5 μM). We also found that these binding partners oligomerize myosin VI, increasing the affinity of oligomerization from >80 μM to 4 μM. We purpose this is achieved by the binding partners relieving auto-inhibition of the cargo-binding domain. Furthermore, we have characterized the interactions of binding partners with full length myosin VI using size-exclusion chromatography and sucrose density gradients. Single molecule photobleaching assays have been performed to determine the stoichiometry of the complexes. Functional measurements have been carried out using ATPase and single molecule motility assays in the presence of the binding partners to determine effects upon the motor activity. Supported by DFG, SFB-863, Freidrich Baur-Stiftung and EMBO." @default.
- W1992004187 created "2016-06-24" @default.
- W1992004187 creator A5002150423 @default.
- W1992004187 creator A5014680070 @default.
- W1992004187 creator A5028187106 @default.
- W1992004187 creator A5051163780 @default.
- W1992004187 creator A5081604457 @default.
- W1992004187 date "2012-01-01" @default.
- W1992004187 modified "2023-09-30" @default.
- W1992004187 title "Myosin VI Comes Together - Structure and Motor Regulation by Binding Partners" @default.
- W1992004187 doi "https://doi.org/10.1016/j.bpj.2011.11.3100" @default.
- W1992004187 hasPublicationYear "2012" @default.
- W1992004187 type Work @default.
- W1992004187 sameAs 1992004187 @default.
- W1992004187 citedByCount "0" @default.
- W1992004187 crossrefType "journal-article" @default.
- W1992004187 hasAuthorship W1992004187A5002150423 @default.
- W1992004187 hasAuthorship W1992004187A5014680070 @default.
- W1992004187 hasAuthorship W1992004187A5028187106 @default.
- W1992004187 hasAuthorship W1992004187A5051163780 @default.
- W1992004187 hasAuthorship W1992004187A5081604457 @default.
- W1992004187 hasBestOaLocation W19920041871 @default.
- W1992004187 hasConcept C107824862 @default.
- W1992004187 hasConcept C121332964 @default.
- W1992004187 hasConcept C12554922 @default.
- W1992004187 hasConcept C160408235 @default.
- W1992004187 hasConcept C170493617 @default.
- W1992004187 hasConcept C181199279 @default.
- W1992004187 hasConcept C185592680 @default.
- W1992004187 hasConcept C20418707 @default.
- W1992004187 hasConcept C23265538 @default.
- W1992004187 hasConcept C28005876 @default.
- W1992004187 hasConcept C31332276 @default.
- W1992004187 hasConcept C51639874 @default.
- W1992004187 hasConcept C55493867 @default.
- W1992004187 hasConcept C62520636 @default.
- W1992004187 hasConcept C6997183 @default.
- W1992004187 hasConcept C86803240 @default.
- W1992004187 hasConcept C91881484 @default.
- W1992004187 hasConcept C93126451 @default.
- W1992004187 hasConcept C95444343 @default.
- W1992004187 hasConceptScore W1992004187C107824862 @default.
- W1992004187 hasConceptScore W1992004187C121332964 @default.
- W1992004187 hasConceptScore W1992004187C12554922 @default.
- W1992004187 hasConceptScore W1992004187C160408235 @default.
- W1992004187 hasConceptScore W1992004187C170493617 @default.
- W1992004187 hasConceptScore W1992004187C181199279 @default.
- W1992004187 hasConceptScore W1992004187C185592680 @default.
- W1992004187 hasConceptScore W1992004187C20418707 @default.
- W1992004187 hasConceptScore W1992004187C23265538 @default.
- W1992004187 hasConceptScore W1992004187C28005876 @default.
- W1992004187 hasConceptScore W1992004187C31332276 @default.
- W1992004187 hasConceptScore W1992004187C51639874 @default.
- W1992004187 hasConceptScore W1992004187C55493867 @default.
- W1992004187 hasConceptScore W1992004187C62520636 @default.
- W1992004187 hasConceptScore W1992004187C6997183 @default.
- W1992004187 hasConceptScore W1992004187C86803240 @default.
- W1992004187 hasConceptScore W1992004187C91881484 @default.
- W1992004187 hasConceptScore W1992004187C93126451 @default.
- W1992004187 hasConceptScore W1992004187C95444343 @default.
- W1992004187 hasIssue "3" @default.
- W1992004187 hasLocation W19920041871 @default.
- W1992004187 hasOpenAccess W1992004187 @default.
- W1992004187 hasPrimaryLocation W19920041871 @default.
- W1992004187 hasRelatedWork W1679408412 @default.
- W1992004187 hasRelatedWork W1992004187 @default.
- W1992004187 hasRelatedWork W1999353363 @default.
- W1992004187 hasRelatedWork W2037970515 @default.
- W1992004187 hasRelatedWork W2089238938 @default.
- W1992004187 hasRelatedWork W2134981881 @default.
- W1992004187 hasRelatedWork W2806983822 @default.
- W1992004187 hasRelatedWork W2914821937 @default.
- W1992004187 hasRelatedWork W4213456994 @default.
- W1992004187 hasRelatedWork W4315754344 @default.
- W1992004187 hasVolume "102" @default.
- W1992004187 isParatext "false" @default.
- W1992004187 isRetracted "false" @default.
- W1992004187 magId "1992004187" @default.
- W1992004187 workType "article" @default.