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- W1992008554 abstract "In the flagellated protozoon Euglena gracilis , characterized nuclear genes harbor atypical introns that usually are flanked by short repeats, adopt complex secondary structures in pre-mRNA, and do not obey the GT-AG rule of conventional cis-spliced introns. In the nuclear fibrillarin gene of E. gracilis , we have identified three spliceosomal-type introns that have GT-AG consensus borders. Furthermore, we have isolated a small RNA from E. gracilis and propose, on the basis of primary and secondary structure comparisons, that it is a homolog of U1 small nuclear RNA, an essential component of the cis-spliceosome in higher eukaryotes. Conserved sequences at the 5′ splice sites of the fibrillarin introns can potentially base pair with Euglena U1 small nuclear RNA. Our observations demonstrate that spliceosomal GT-AG cis-splicing occurs in Euglena , in addition to the nonconventional cis-splicing and spliced leader trans-splicing previously recognized in this early diverging unicellular eukaryote." @default.
- W1992008554 created "2016-06-24" @default.
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- W1992008554 date "1999-02-02" @default.
- W1992008554 modified "2023-09-25" @default.
- W1992008554 title "U1 small nuclear RNA and spliceosomal introns in <i>Euglena</i> <i>gracilis</i>" @default.
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- W1992008554 doi "https://doi.org/10.1073/pnas.96.3.852" @default.
- W1992008554 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/15314" @default.
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