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- W1992022039 abstract "In this work, we reported a simple rapid and point-of-care magnetic immunofluorescence assay for avian influenza virus (AIV) and developed a portable experimental setup equipped with an optical fiber spectrometer and a microfluidic device. We achieved the integration of immunomagnetic target capture, concentration, and fluorescence detection in the microfluidic chip. By optimizing flow rate and incubation time, we could get a limit of detection low up to 3.7 × 10(4) copy/μL with a sample consumption of 2 μL and a total assay time of less than 55 min. This approach had proved to possess high portability, fast analysis, high specificity, high precision, and reproducibility with an intra-assay variability of 2.87% and an interassay variability of 4.36%. As a whole, this microfluidic system may provide a powerful platform for the rapid detection of AIV and may be extended for detection of other viral pathogens; in addition, this portable experimental setup enables the development of point-of-care diagnostic systems while retaining adequate sensitivity." @default.
- W1992022039 created "2016-06-24" @default.
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- W1992022039 date "2013-02-22" @default.
- W1992022039 modified "2023-10-01" @default.
- W1992022039 title "A Simple Point-of-Care Microfluidic Immunomagnetic Fluorescence Assay for Pathogens" @default.
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- W1992022039 doi "https://doi.org/10.1021/ac302903p" @default.
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