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• W1992025963 abstract "Three seed lots, obtained from different ‘foundation farms’, for each of the alfalfa, Medicago sativa L., ecotypes ‘Vogherese’ and ‘Maremmana’, together with the variety ‘Iside’ as a control, were studied to test the possibility of distinguishing them through restriction fragment length polymorphism analysis. Twenty plants per seed lot were analysed with 25 DNA probes that yielded 155 informative polymorphic fragments. Variance partitioning showed that within-population variability accounted for nearly 98% of the total variance, while a small but significant contribution to the total variance was due to among-lot variability.‘Vogherese’ was more homogeneous than ‘Maremmana’, as a consequence of the greater environmental homogeneity of the adaptation area of the former ecotype compared with the latter. Bulk analysis yielded eight variety-specific bands, 12 bands present in both ecotypes and absent in the variety and one band specific of ‘Maremmana’. Results are discussed in relation to the practical use of molecular markers for alfalfa ecotype identification." @default.
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• W1992025963 date "2000-07-01" @default.
• W1992025963 modified "2023-09-26" @default.
• W1992025963 title "RFLP analysis allows for the identification of alfalfa ecotypes" @default.
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• W1992025963 doi "https://doi.org/10.1046/j.1439-0523.2000.00478.x" @default.
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