Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992029307> ?p ?o ?g. }
- W1992029307 endingPage "20140009" @default.
- W1992029307 startingPage "20140009" @default.
- W1992029307 abstract "Stem cells receive numerous cues from their associated substrate that help to govern their behaviour. However, identification of influential substrate characteristics poses difficulties because of their complex nature. In this study, we developed an integrated experimental and systems level modelling approach to investigate and identify specific substrate features influencing differentiation of mouse embryonic stem cells (mESCs) on a model fibrous substrate, fibrin. We synthesized a range of fibrin gels by varying fibrinogen and thrombin concentrations, which led to a range of substrate stiffness and microstructure. mESCs were cultured on each of these gels, and characterization of the differentiated cells revealed a strong influence of substrate modulation on gene expression patterning. To identify specific substrate features influencing differentiation, the substrate microstructure was quantified by image analysis and correlated with stem cell gene expression patterns using a statistical model. Significant correlations were observed between differentiation and microstructure features, specifically fibre alignment. Furthermore, this relationship occurred in a lineage-specific manner towards endoderm. This systems level approach allows for identification of specific substrate features from a complex material which are influential to cellular behaviour. Such analysis may be effective in guiding the design of scaffolds with specific properties for tissue engineering applications." @default.
- W1992029307 created "2016-06-24" @default.
- W1992029307 creator A5013192471 @default.
- W1992029307 creator A5015726446 @default.
- W1992029307 creator A5023837738 @default.
- W1992029307 creator A5032755922 @default.
- W1992029307 creator A5045386624 @default.
- W1992029307 creator A5067981873 @default.
- W1992029307 creator A5087175427 @default.
- W1992029307 creator A5090003388 @default.
- W1992029307 date "2014-06-06" @default.
- W1992029307 modified "2023-10-01" @default.
- W1992029307 title "Systems level approach reveals the correlation of endoderm differentiation of mouse embryonic stem cells with specific microstructural cues of fibrin gels" @default.
- W1992029307 cites W1480376833 @default.
- W1992029307 cites W1603072500 @default.
- W1992029307 cites W1617770442 @default.
- W1992029307 cites W1837441996 @default.
- W1992029307 cites W1951289170 @default.
- W1992029307 cites W1965419330 @default.
- W1992029307 cites W1966955307 @default.
- W1992029307 cites W1970041028 @default.
- W1992029307 cites W1980943128 @default.
- W1992029307 cites W1988059567 @default.
- W1992029307 cites W199040808 @default.
- W1992029307 cites W1997777374 @default.
- W1992029307 cites W1999484255 @default.
- W1992029307 cites W2001009280 @default.
- W1992029307 cites W2002095196 @default.
- W1992029307 cites W2002128589 @default.
- W1992029307 cites W2003251335 @default.
- W1992029307 cites W2004754771 @default.
- W1992029307 cites W2007142798 @default.
- W1992029307 cites W2007260996 @default.
- W1992029307 cites W2008289938 @default.
- W1992029307 cites W2010227280 @default.
- W1992029307 cites W2013926604 @default.
- W1992029307 cites W2014672025 @default.
- W1992029307 cites W2015979204 @default.
- W1992029307 cites W2022336190 @default.
- W1992029307 cites W2038023628 @default.
- W1992029307 cites W2042022714 @default.
- W1992029307 cites W2044021612 @default.
- W1992029307 cites W2049718692 @default.
- W1992029307 cites W2052540803 @default.
- W1992029307 cites W2054361177 @default.
- W1992029307 cites W2056423944 @default.
- W1992029307 cites W2059746822 @default.
- W1992029307 cites W2062118486 @default.
- W1992029307 cites W2063167928 @default.
- W1992029307 cites W2068128154 @default.
- W1992029307 cites W2069271003 @default.
- W1992029307 cites W2071105861 @default.
- W1992029307 cites W2071219878 @default.
- W1992029307 cites W2071553413 @default.
- W1992029307 cites W2074607580 @default.
- W1992029307 cites W2078024459 @default.
- W1992029307 cites W2079508597 @default.
- W1992029307 cites W2083684354 @default.
- W1992029307 cites W2086943348 @default.
- W1992029307 cites W2090237060 @default.
- W1992029307 cites W2121472082 @default.
- W1992029307 cites W2122033285 @default.
- W1992029307 cites W2122579194 @default.
- W1992029307 cites W2126598930 @default.
- W1992029307 cites W2129128296 @default.
- W1992029307 cites W2134208433 @default.
- W1992029307 cites W2138442302 @default.
- W1992029307 cites W2140712402 @default.
- W1992029307 cites W2141738357 @default.
- W1992029307 cites W2145854069 @default.
- W1992029307 cites W2162944563 @default.
- W1992029307 cites W2163367446 @default.
- W1992029307 cites W2163784056 @default.
- W1992029307 cites W2165122639 @default.
- W1992029307 cites W2168967248 @default.
- W1992029307 cites W2169755701 @default.
- W1992029307 cites W2172237567 @default.
- W1992029307 cites W2316326431 @default.
- W1992029307 doi "https://doi.org/10.1098/rsif.2014.0009" @default.
- W1992029307 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4006239" @default.
- W1992029307 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24718448" @default.
- W1992029307 hasPublicationYear "2014" @default.
- W1992029307 type Work @default.
- W1992029307 sameAs 1992029307 @default.
- W1992029307 citedByCount "12" @default.
- W1992029307 countsByYear W19920293072014 @default.
- W1992029307 countsByYear W19920293072015 @default.
- W1992029307 countsByYear W19920293072016 @default.
- W1992029307 countsByYear W19920293072017 @default.
- W1992029307 countsByYear W19920293072018 @default.
- W1992029307 countsByYear W19920293072019 @default.
- W1992029307 countsByYear W19920293072020 @default.
- W1992029307 countsByYear W19920293072022 @default.
- W1992029307 crossrefType "journal-article" @default.
- W1992029307 hasAuthorship W1992029307A5013192471 @default.
- W1992029307 hasAuthorship W1992029307A5015726446 @default.
- W1992029307 hasAuthorship W1992029307A5023837738 @default.
- W1992029307 hasAuthorship W1992029307A5032755922 @default.