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• W1992033675 startingPage "749" @default.
• W1992033675 abstract "Reporter gene technology is widely used to monitor the cellular events associated with signal transduction and gene expression. Based upon the splicing of transcriptional control elements to a variety of reporter genes (with easily measurable phenotypes), it “reports” the effects of a cascade of signalling events on gene expression inside cells. The principal advantage of these assays is their high sensitivity, reliability, convenience, and adaptability to large-scale measurements. This review summarises the current status of reporter gene technology including its role in monitoring gene transfer and expression and its development as a biological screen. With the advances in this technology and in detection methods, it is likely that luciferase and green fluorescent protein will become increasingly popular for the non-invasive monitoring of gene expression in living tissues and cells. Such techniques will be important in defining the molecular events associated with gene transcription, which has implications for our understanding of the molecular basis of disease and will influence our approach to gene therapy and drug development." @default.
• W1992033675 created "2016-06-24" @default.
• W1992033675 creator A5010184957 @default.
• W1992033675 date "1999-09-01" @default.
• W1992033675 modified "2023-10-10" @default.
• W1992033675 title "Reporter gene technology: the future looks bright" @default.
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