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• W1992038424 abstract "Aims The induction of tumour cell death by apoptosis is a major goal of cancer therapy and the in situ detection of apoptosis in tumour tissue has become an important diagnostic parameter. Different apoptosis detection methods assess distinct biochemical processes in the dying cell. Thus, their direct comparison is mandatory to evaluate their diagnostic value. The aim of this study was to compare the immunohistochemical detection of active caspase 3 and single-stranded DNA in primary and metastatic liver tumours as markers of apoptotic cell death. Methods We studied detection of active caspase 3 and single-stranded DNA in 20 primary hepatocellular carcinomas (HCC) and 20 liver metastases from colorectal carcinomas (CRC) using immunohistochemistry on paraffin sections. Results Our results reveal that both methods are suitable and sensitive techniques for the in situ detection of apoptosis, however, they also demonstrate that immunohis- tochemistry for active caspase 3 and single-stranded DNA have differential sensitivities in HCC and CRC. Conclusion The sensitivity of apoptosis detection using immunohistochemistry for active caspase 3 and singlestranded DNA may be tumour cell type dependent." @default.
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• W1992038424 date "2007-12-01" @default.
• W1992038424 modified "2023-10-16" @default.
• W1992038424 title "Active caspase 3 and DNA fragmentation as markers for apoptotic cell death in primary and metastatic liver tumours" @default.
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• W1992038424 doi "https://doi.org/10.1080/00313020701684375" @default.
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