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- W1992046557 abstract "Abstract Lipase hydrolysis was evaluated as a means of selectively enriching long‐chain ω3 fatty acids in fish oil. Several lipases were screened for their ability to enrich total ω‐3 acids or selectively enrich either docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA). The effect of enzyme concentration, degree of hydrolysis, and fatty acid composition of the feed oil was studied. Because the materials that were enriched in long‐chain ω3 acids were either partial glycerides or free fatty acids, enzymatic reesterification of these materials to triglycerides by lipase catalysis was also investigated. Hydrolysis of fish oil by either Candida rugosa or Geotrichum candidum lipases resulted in an increase in the content of total ω3 acids from about 30% in the feed oil to 45% in the partial glycerides. The lipase from C. rugosa was effective in selectively enriching either DHA or EPA, resulting in a change of either the DHA/EPA ratio or the EPA/DHA ratio from approximately 1:1 to 5:1. Nonselective reesterification of free fatty acids or partial glycerides that contained ω3 fatty acids could be achieved at high efficiency (approximately 95% triglycerides in the product) by using immobilized Rhizomucor miehei lipase with continuous removal of water." @default.
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- W1992046557 date "1996-11-01" @default.
- W1992046557 modified "2023-10-15" @default.
- W1992046557 title "Lipase‐catalyzed enrichment of long‐chain polyunsaturated fatty acids" @default.
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- W1992046557 doi "https://doi.org/10.1007/bf02523503" @default.
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