Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992049899> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1992049899 endingPage "2129" @default.
- W1992049899 startingPage "2123" @default.
- W1992049899 abstract "A sensitive, selective, and quantitative method for the simultaneous determination of gemcitabine and 2,2-difluoro-2-deoxyuridine (dFdU) has been developed and validated in human plasma in the presence of tetrahydrouridine, a cytidine deaminase inhibitor. The method employs derivatization of gemcitabine and dFdU with dansyl chloride to improve the chromatographic retention and separation. The derivatization was performed in plasma without prior sample clean-up, followed by extraction of the dansyl-derivatives using methyl tertiary-butyl ether (MTBE). Ultra performance liquid chromatography (UPLC) technology on a BEH C18 stationary phase column with 1.7 μm particle size was used for chromatographic separation coupled to tandem mass spectrometry. The method was validated over the concentration ranges of 20–5000 and 100–25,000 ng/mL for gemcitabine and dFdU, respectively. The results from assay validation show that the method is rugged, precise, accurate, and well-suited to support pharmacokinetic studies. In addition, the relatively small sample volume (50 μL) and a run time of 1.5 min facilitate automation and allow for high-throughput analysis." @default.
- W1992049899 created "2016-06-24" @default.
- W1992049899 creator A5012736318 @default.
- W1992049899 creator A5013291304 @default.
- W1992049899 creator A5070574885 @default.
- W1992049899 date "2009-07-01" @default.
- W1992049899 modified "2023-10-15" @default.
- W1992049899 title "Development of a sensitive and selective LC–MS/MS method for simultaneous determination of gemcitabine and 2,2-difluoro-2-deoxyuridine in human plasma" @default.
- W1992049899 cites W1890647543 @default.
- W1992049899 cites W1898871412 @default.
- W1992049899 cites W1973681303 @default.
- W1992049899 cites W1977515504 @default.
- W1992049899 cites W1991045666 @default.
- W1992049899 cites W2003463247 @default.
- W1992049899 cites W2024004800 @default.
- W1992049899 cites W2037466436 @default.
- W1992049899 cites W2040560614 @default.
- W1992049899 cites W2045149855 @default.
- W1992049899 cites W2059870291 @default.
- W1992049899 cites W2074868392 @default.
- W1992049899 cites W2082546523 @default.
- W1992049899 cites W2083803599 @default.
- W1992049899 cites W2093004194 @default.
- W1992049899 cites W2093447536 @default.
- W1992049899 cites W2096868619 @default.
- W1992049899 cites W2101465269 @default.
- W1992049899 cites W2135551003 @default.
- W1992049899 cites W2143375202 @default.
- W1992049899 cites W2154587211 @default.
- W1992049899 cites W2160812793 @default.
- W1992049899 cites W2166574172 @default.
- W1992049899 doi "https://doi.org/10.1016/j.jchromb.2009.06.002" @default.
- W1992049899 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19546035" @default.
- W1992049899 hasPublicationYear "2009" @default.
- W1992049899 type Work @default.
- W1992049899 sameAs 1992049899 @default.
- W1992049899 citedByCount "51" @default.
- W1992049899 countsByYear W19920498992012 @default.
- W1992049899 countsByYear W19920498992013 @default.
- W1992049899 countsByYear W19920498992015 @default.
- W1992049899 countsByYear W19920498992017 @default.
- W1992049899 countsByYear W19920498992018 @default.
- W1992049899 countsByYear W19920498992019 @default.
- W1992049899 countsByYear W19920498992020 @default.
- W1992049899 countsByYear W19920498992021 @default.
- W1992049899 countsByYear W19920498992022 @default.
- W1992049899 countsByYear W19920498992023 @default.
- W1992049899 crossrefType "journal-article" @default.
- W1992049899 hasAuthorship W1992049899A5012736318 @default.
- W1992049899 hasAuthorship W1992049899A5013291304 @default.
- W1992049899 hasAuthorship W1992049899A5070574885 @default.
- W1992049899 hasConcept C179998833 @default.
- W1992049899 hasConcept C185592680 @default.
- W1992049899 hasConcept C2776923573 @default.
- W1992049899 hasConcept C2992520659 @default.
- W1992049899 hasConcept C43617362 @default.
- W1992049899 hasConceptScore W1992049899C179998833 @default.
- W1992049899 hasConceptScore W1992049899C185592680 @default.
- W1992049899 hasConceptScore W1992049899C2776923573 @default.
- W1992049899 hasConceptScore W1992049899C2992520659 @default.
- W1992049899 hasConceptScore W1992049899C43617362 @default.
- W1992049899 hasIssue "22" @default.
- W1992049899 hasLocation W19920498991 @default.
- W1992049899 hasLocation W19920498992 @default.
- W1992049899 hasOpenAccess W1992049899 @default.
- W1992049899 hasPrimaryLocation W19920498991 @default.
- W1992049899 hasRelatedWork W1590758024 @default.
- W1992049899 hasRelatedWork W2019884116 @default.
- W1992049899 hasRelatedWork W2026391790 @default.
- W1992049899 hasRelatedWork W2038405227 @default.
- W1992049899 hasRelatedWork W2039848115 @default.
- W1992049899 hasRelatedWork W2058645934 @default.
- W1992049899 hasRelatedWork W2095219531 @default.
- W1992049899 hasRelatedWork W2884775158 @default.
- W1992049899 hasRelatedWork W4256546821 @default.
- W1992049899 hasRelatedWork W4323903764 @default.
- W1992049899 hasVolume "877" @default.
- W1992049899 isParatext "false" @default.
- W1992049899 isRetracted "false" @default.
- W1992049899 magId "1992049899" @default.
- W1992049899 workType "article" @default.