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- W1992050169 abstract "DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 71:175-178 (2006) - doi:10.3354/dao071175 Effect of hyperthermia on the replication of white spot syndrome virus (WSSV) in Procambarus clarkii Hua-Hua Du, Wei-Fen Li, Zi-Rong Xu*, Zong-Su Kil Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Feed Science Institute, Zhejiang University, Hangzhou 310029, PR China *Corresponding author. Email: zrxu@zju.edu.cn ABSTRACT: The effect of hyperthermia on the development of white spot syndrome virus (WSSV) in the crayfish Procambarus clarkii was studied by competitive PCR. Crayfish were exposed to different temperatures (24 ± 1 and 32 ± 1°C) after WSSV injection. No mortality was observed when crayfish were held at 32 ± 1°C, but mortality reached 100% when crayfish were transferred to 24 ± 1°C. Competitive PCR showed that viral levels at 32 ± 1°C remained at 105 copies mg1 tissue, while at 24 ± 1°C levels were significantly higher, rising from 104 to 1010 copies mg1 tissue. These results suggest that hyperthermia reduces viral replication, but does not eliminate viral particles from WSSV-infected crayfish. KEY WORDS: White spot syndrome virus · Hyperthermia · Viral replication · Procambarus clarkii · Competitive PCR · Water temperature Full text in pdf format PreviousExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 71, No. 2. Online publication date: July 25, 2006 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2006 Inter-Research." @default.
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- W1992050169 title "Effect of hyperthermia on the replication of white spot syndrome virus (WSSV) in Procambarus clarkii" @default.
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