FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992078366> ?p ?o ?g. }

• W1992078366 endingPage "e83607" @default.
• W1992078366 startingPage "e83607" @default.
• W1992078366 abstract "Selection of lung cancer patients for therapy with tyrosine kinase inhibitors directed at EGFR requires the identification of specific EGFR mutations. In most patients with advanced, inoperable lung carcinoma limited tumor samples often represent the only material available for both histologic typing and molecular analysis. We defined a next generation sequencing protocol targeted to EGFR exons 18-21 suitable for the routine diagnosis of such clinical samples. The protocol was validated in an unselected series of 80 small biopsies (n=14) and cytology (n=66) specimens representative of the material ordinarily submitted for diagnostic evaluation to three referral medical centers in Italy. Specimens were systematically evaluated for tumor cell number and proportion relative to non-neoplastic cells. They were analyzed in batches of 100-150 amplicons per run, reaching an analytical sensitivity of 1% and obtaining an adequate number of reads, to cover all exons on all samples analyzed. Next generation sequencing was compared with Sanger sequencing. The latter identified 15 EGFR mutations in 14/80 cases (17.5%) but did not detected mutations when the proportion of neoplastic cells was below 40%. Next generation sequencing identified 31 EGFR mutations in 24/80 cases (30.0%). Mutations were detected with a proportion of neoplastic cells as low as 5%. All mutations identified by the Sanger method were confirmed. In 6 cases next generation sequencing identified exon 19 deletions or the L858R mutation not seen after Sanger sequencing, allowing the patient to be treated with tyrosine kinase inhibitors. In one additional case the R831H mutation associated with treatment resistance was identified in an EGFR wild type tumor after Sanger sequencing. Next generation sequencing is robust, cost-effective and greatly improves the detection of EGFR mutations. Its use should be promoted for the clinical diagnosis of mutations in specimens with unfavorable tumor cell content." @default.
• W1992078366 created "2016-06-24" @default.
• W1992078366 creator A5017669867 @default.
• W1992078366 creator A5032330087 @default.
• W1992078366 creator A5042347653 @default.
• W1992078366 creator A5049212835 @default.
• W1992078366 creator A5054390661 @default.
• W1992078366 creator A5055732365 @default.
• W1992078366 creator A5057019569 @default.
• W1992078366 creator A5083170563 @default.
• W1992078366 creator A5085931728 @default.
• W1992078366 creator A5089411528 @default.
• W1992078366 date "2013-12-23" @default.
• W1992078366 modified "2023-10-13" @default.
• W1992078366 title "Next-Generation Sequencing of Lung Cancer EGFR Exons 18-21 Allows Effective Molecular Diagnosis of Small Routine Samples (Cytology and Biopsy)" @default.
• W1992078366 cites W1539990701 @default.
• W1992078366 cites W1569610737 @default.
• W1992078366 cites W1653677725 @default.
• W1992078366 cites W1922786366 @default.
• W1992078366 cites W1965856472 @default.
• W1992078366 cites W1966110952 @default.
• W1992078366 cites W1968458420 @default.
• W1992078366 cites W1970433797 @default.
• W1992078366 cites W1970991186 @default.
• W1992078366 cites W1979332174 @default.
• W1992078366 cites W1989188414 @default.
• W1992078366 cites W2002937040 @default.
• W1992078366 cites W2006384917 @default.
• W1992078366 cites W2013733453 @default.
• W1992078366 cites W2030883899 @default.
• W1992078366 cites W2036792965 @default.
• W1992078366 cites W2037353038 @default.
• W1992078366 cites W2042537546 @default.
• W1992078366 cites W2043696829 @default.
• W1992078366 cites W2046871652 @default.
• W1992078366 cites W2048293465 @default.
• W1992078366 cites W2054636737 @default.
• W1992078366 cites W2060987657 @default.
• W1992078366 cites W2070396662 @default.
• W1992078366 cites W2077437377 @default.
• W1992078366 cites W2079294958 @default.
• W1992078366 cites W2083829591 @default.
• W1992078366 cites W2085277706 @default.
• W1992078366 cites W2089716357 @default.
• W1992078366 cites W2092950092 @default.
• W1992078366 cites W2095618958 @default.
• W1992078366 cites W2096545560 @default.
• W1992078366 cites W2096786080 @default.
• W1992078366 cites W2103388071 @default.
• W1992078366 cites W2111662961 @default.
• W1992078366 cites W2112096254 @default.
• W1992078366 cites W2118526609 @default.
• W1992078366 cites W2120669034 @default.
• W1992078366 cites W2124313184 @default.
• W1992078366 cites W2129360604 @default.
• W1992078366 cites W2130254323 @default.
• W1992078366 cites W2131831783 @default.
• W1992078366 cites W2132157071 @default.
• W1992078366 cites W2133784891 @default.
• W1992078366 cites W2135192471 @default.
• W1992078366 cites W2140126391 @default.
• W1992078366 cites W2140775102 @default.
• W1992078366 cites W2142850084 @default.
• W1992078366 cites W2147703884 @default.
• W1992078366 cites W2156713238 @default.
• W1992078366 cites W2158667695 @default.
• W1992078366 cites W2160982674 @default.
• W1992078366 cites W2161821474 @default.
• W1992078366 cites W2162645837 @default.
• W1992078366 cites W2163195607 @default.
• W1992078366 cites W2165762354 @default.
• W1992078366 cites W2166084034 @default.
• W1992078366 cites W2166373066 @default.
• W1992078366 cites W2168156874 @default.
• W1992078366 cites W2168930524 @default.
• W1992078366 cites W2169215908 @default.
• W1992078366 cites W2316339075 @default.
• W1992078366 cites W2514360855 @default.
• W1992078366 doi "https://doi.org/10.1371/journal.pone.0083607" @default.
• W1992078366 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3871524" @default.
• W1992078366 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24376723" @default.
• W1992078366 hasPublicationYear "2013" @default.
• W1992078366 type Work @default.
• W1992078366 sameAs 1992078366 @default.
• W1992078366 citedByCount "71" @default.
• W1992078366 countsByYear W19920783662014 @default.
• W1992078366 countsByYear W19920783662015 @default.
• W1992078366 countsByYear W19920783662016 @default.
• W1992078366 countsByYear W19920783662017 @default.
• W1992078366 countsByYear W19920783662018 @default.
• W1992078366 countsByYear W19920783662019 @default.
• W1992078366 countsByYear W19920783662020 @default.
• W1992078366 countsByYear W19920783662021 @default.
• W1992078366 countsByYear W19920783662022 @default.
• W1992078366 crossrefType "journal-article" @default.
• W1992078366 hasAuthorship W1992078366A5017669867 @default.
• W1992078366 hasAuthorship W1992078366A5032330087 @default.
• W1992078366 hasAuthorship W1992078366A5042347653 @default.

• next