FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992078963> ?p ?o ?g. }

• W1992078963 endingPage "2806" @default.
• W1992078963 startingPage "2797" @default.
• W1992078963 abstract "The ability of the human heme oxygenase-1 (hHO-1) R183E mutant to oxidize heme in reactions supported by either NADPH-cytochrome P450 reductase or ascorbic acid has been compared. The NADPH-dependent reaction, like that of wild-type hHO-1, yields exclusively biliverdin IXα. In contrast, the R183E mutant with ascorbic acid as the reductant produces biliverdin IXα (79 ± 4%), IXδ (19 ± 3%), and a trace of IXβ. In the presence of superoxide dismutase and catalase, the yield of biliverdin IXδ is decreased to 8 ± 1% with a corresponding increase in biliverdin IXα. Spectroscopic analysis of the NADPH-dependent reaction shows that the R183E ferric biliverdin complex accumulates, because reduction of the iron, which is required for sequential iron and biliverdin release, is impaired. Reversal of the charge at position 183 makes reduction of the iron more difficult. The crystal structure of the R183E mutant, determined in the ferric and ferrous-NO bound forms, shows that the heme primarily adopts the same orientation as in wild-type hHO-1. The structure of the Fe(II)·NO complex suggests that an altered active site hydrogen bonding network supports catalysis in the R183E mutant. Furthermore, Arg-183 contributes to the regiospecificity of the wild-type enzyme, but its contribution is not critical. The results indicate that the ascorbate-dependent reaction is subject to a lower degree of regiochemical control than the NADPH-dependent reaction. Ascorbate may be able to reduce the R183E ferric and ferrous dioxygen complexes in active site conformations that cannot be reduced by NADPH-cytochrome P450 reductase." @default.
• W1992078963 created "2016-06-24" @default.
• W1992078963 creator A5003621282 @default.
• W1992078963 creator A5009013274 @default.
• W1992078963 creator A5015794500 @default.
• W1992078963 creator A5070426242 @default.
• W1992078963 date "2005-01-01" @default.
• W1992078963 modified "2023-10-10" @default.
• W1992078963 title "Regiospecificity Determinants of Human Heme Oxygenase" @default.
• W1992078963 cites W147446370 @default.
• W1992078963 cites W1487329487 @default.
• W1992078963 cites W1561190910 @default.
• W1992078963 cites W1572334445 @default.
• W1992078963 cites W1586487499 @default.
• W1992078963 cites W1600690434 @default.
• W1992078963 cites W1922292712 @default.
• W1992078963 cites W1964925554 @default.
• W1992078963 cites W1966431834 @default.
• W1992078963 cites W1966582616 @default.
• W1992078963 cites W1970675158 @default.
• W1992078963 cites W1973081785 @default.
• W1992078963 cites W1978765308 @default.
• W1992078963 cites W1980564886 @default.
• W1992078963 cites W1984216393 @default.
• W1992078963 cites W1984386284 @default.
• W1992078963 cites W1986191025 @default.
• W1992078963 cites W1986568904 @default.
• W1992078963 cites W1992434069 @default.
• W1992078963 cites W1995017064 @default.
• W1992078963 cites W1998412842 @default.
• W1992078963 cites W2000370758 @default.
• W1992078963 cites W2006380614 @default.
• W1992078963 cites W2010262048 @default.
• W1992078963 cites W2020941408 @default.
• W1992078963 cites W2021986872 @default.
• W1992078963 cites W2024416107 @default.
• W1992078963 cites W2028231353 @default.
• W1992078963 cites W2038700761 @default.
• W1992078963 cites W2039679742 @default.
• W1992078963 cites W2044916014 @default.
• W1992078963 cites W2047119967 @default.
• W1992078963 cites W2056523101 @default.
• W1992078963 cites W2060174475 @default.
• W1992078963 cites W2066484244 @default.
• W1992078963 cites W2067141031 @default.
• W1992078963 cites W2071291311 @default.
• W1992078963 cites W2077587761 @default.
• W1992078963 cites W2080528351 @default.
• W1992078963 cites W2086861371 @default.
• W1992078963 cites W2094122546 @default.
• W1992078963 cites W2103314026 @default.
• W1992078963 cites W2134374955 @default.
• W1992078963 cites W2143009084 @default.
• W1992078963 cites W2168427863 @default.
• W1992078963 doi "https://doi.org/10.1074/jbc.m411229200" @default.
• W1992078963 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15525643" @default.
• W1992078963 hasPublicationYear "2005" @default.
• W1992078963 type Work @default.
• W1992078963 sameAs 1992078963 @default.
• W1992078963 citedByCount "18" @default.
• W1992078963 countsByYear W19920789632013 @default.
• W1992078963 countsByYear W19920789632014 @default.
• W1992078963 countsByYear W19920789632019 @default.
• W1992078963 countsByYear W19920789632021 @default.
• W1992078963 countsByYear W19920789632022 @default.
• W1992078963 countsByYear W19920789632023 @default.
• W1992078963 crossrefType "journal-article" @default.
• W1992078963 hasAuthorship W1992078963A5003621282 @default.
• W1992078963 hasAuthorship W1992078963A5009013274 @default.
• W1992078963 hasAuthorship W1992078963A5015794500 @default.
• W1992078963 hasAuthorship W1992078963A5070426242 @default.
• W1992078963 hasBestOaLocation W19920789631 @default.
• W1992078963 hasConcept C165474305 @default.
• W1992078963 hasConcept C181199279 @default.
• W1992078963 hasConcept C185592680 @default.
• W1992078963 hasConcept C2776217839 @default.
• W1992078963 hasConcept C2779219270 @default.
• W1992078963 hasConcept C55493867 @default.
• W1992078963 hasConceptScore W1992078963C165474305 @default.
• W1992078963 hasConceptScore W1992078963C181199279 @default.
• W1992078963 hasConceptScore W1992078963C185592680 @default.
• W1992078963 hasConceptScore W1992078963C2776217839 @default.
• W1992078963 hasConceptScore W1992078963C2779219270 @default.
• W1992078963 hasConceptScore W1992078963C55493867 @default.
• W1992078963 hasIssue "4" @default.
• W1992078963 hasLocation W19920789631 @default.
• W1992078963 hasOpenAccess W1992078963 @default.
• W1992078963 hasPrimaryLocation W19920789631 @default.
• W1992078963 hasRelatedWork W113801019 @default.
• W1992078963 hasRelatedWork W1966948929 @default.
• W1992078963 hasRelatedWork W2001854543 @default.
• W1992078963 hasRelatedWork W2015100043 @default.
• W1992078963 hasRelatedWork W2021046573 @default.
• W1992078963 hasRelatedWork W2068052817 @default.
• W1992078963 hasRelatedWork W2074733139 @default.
• W1992078963 hasRelatedWork W2079610230 @default.
• W1992078963 hasRelatedWork W2092330256 @default.
• W1992078963 hasRelatedWork W2360170688 @default.

• next