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- W1992079489 abstract "A collagen-mimetic polymer that can be easily engineered with specific cell-responsive and mechanical properties would be of significant interest for fundamental cell-matrix studies and applications in regenerative medicine. However, oligonucleotide-based synthesis of full-length collagen has been encumbered by the characteristic glycine-X-Y sequence repetition, which promotes mismatched oligonucleotide hybridizations during de novo gene assembly. In this work, we report a novel, modular synthesis strategy that yields full-length human collagen III and specifically defined variants. We used a computational algorithm that applies codon degeneracy to design oligonucleotides that favor correct hybridizations while disrupting incorrect ones for gene synthesis. The resulting recombinant polymers were expressed in Saccharomyces cerevisiae engineered with prolyl-4-hydroxylase. Our modular approach enabled mixing-and-matching domains to fabricate different combinations of collagen variants that contained different secretion signals at the N-terminus and cysteine residues imbedded within the triple-helical domain at precisely defined locations. This work shows the flexibility of our strategy for designing and assembling specifically tailored biomimetic collagen polymers with re-engineered properties." @default.
- W1992079489 created "2016-06-24" @default.
- W1992079489 creator A5028009303 @default.
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- W1992079489 date "2010-05-18" @default.
- W1992079489 modified "2023-10-14" @default.
- W1992079489 title "Recombinant Human Collagen and Biomimetic Variants Using a De Novo Gene Optimized for Modular Assembly" @default.
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- W1992079489 cites W1967390330 @default.
- W1992079489 cites W1969215799 @default.
- W1992079489 cites W1969590456 @default.
- W1992079489 cites W1969946367 @default.
- W1992079489 cites W1970525947 @default.
- W1992079489 cites W1970576706 @default.
- W1992079489 cites W1975422671 @default.
- W1992079489 cites W1976958546 @default.
- W1992079489 cites W1977566832 @default.
- W1992079489 cites W1977840710 @default.
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- W1992079489 cites W1980225254 @default.
- W1992079489 cites W1982769493 @default.
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- W1992079489 doi "https://doi.org/10.1021/bm100052y" @default.
- W1992079489 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20481478" @default.
- W1992079489 hasPublicationYear "2010" @default.
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