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- W1992103313 abstract "A copolymer of 50% ethylene oxide and 50% propylene oxide (EOPO(1:1) ) has been used in aqueous two-phase partitioning of amino acids. This EOPO(1:1) copolymer has a cloud point of 70 °C, at which it separates from water. The cloud point can be lowered to 45 °C by the addition of 0.2 M sodium sulfate. Amino acids are first partitioned at 25 °C in a system composed of EOPO(1:1) and hydroxypropyl starch (PES) . EOPO(1:1) is enriched in the top phase and PES is in the bottom phase. Then, the top phase can be removed and its temperature increases above the cloud point of the EOPO(1:1) . This results in formation of a new two-phase system with a top aqueous phase containing most of the amino acids and a bottom EOPO(1:1) -rich phase, thus allowing the lower EOPO(1:1) -rich phase to be removed and recycled for a second extraction. Amino acids are recovered exclusively in top aqueous phase." @default.
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- W1992103313 date "1997-02-05" @default.
- W1992103313 modified "2023-09-23" @default.
- W1992103313 title "Partitioning of Amino Acids by Aqueous Two-Phase Systems Combined with Temperature-Induced Phase Formation" @default.
- W1992103313 doi "https://doi.org/10.1021/bp9600750" @default.
- W1992103313 hasPublicationYear "1997" @default.
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