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- W1992150382 abstract "Three approaches to the synthesis of the repetitive copolypeptide [-(GlyAla)3- GlyProGlu-]n (1) are described. Direct chemical synthesis of 1 via classical solution methods required 18 steps and afforded a polydisperse product with an average molecular weight of less than 10 000. Two alternative genetic strategies were also explored. In the first, chemically synthesized DNA oligomers were self-ligated to produce a population of multimers, which were fitted with translational start and stop signals and inserted into an expression plasmid containing the λ PL promoter and a synthetic ribosome binding site. Transformation of E. coli led to the isolation of a stable recombinant plasmid carrying an insert encoding 12 repeats of sequence 1. Attempts to identify polypeptide 1 after induction of transformed cultures were unsuccessful. A second strategy, generating a tripartite derivative of sequence 1 carrying short N-and C-terminal extensions, afforded excellent yields of product. The relative merits of chemical and genetic approaches to repetitive polypeptide materials are discussed." @default.
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- W1992150382 date "1990-05-01" @default.
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- W1992150382 title "Chemical and biosynthetic approaches to the production of novel polypeptide materials" @default.
- W1992150382 doi "https://doi.org/10.1021/bp00003a004" @default.
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