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- W1992201149 abstract "Using open-top chambers, the impact of ozone (O(3)) on foliar carboxylases of bean (Phaseolus vulgaris L.) was investigated. From sowing, beans were exposed to non-filtered air (NF) and NF supplied with 40 (+40) and 80 (+80) nL L(-1) O(3). Twenty days after emergence, primary and first trifoliate leaves were sampled. Biochemical characteristics of leaves from +40 were quite similar to those from NF. Conversely, +80 induced distinct biochemical effects in primary and first trifoliate leaves. Regarding primary leaves, +80 reduced ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity by 33% whereas it stimulated phosphoenolpyruvate carboxylase (PEPc) activity by 376%. The reduction in Rubisco activity was accompanied by a decrease in both Rubisco subunit amounts and a consistent oxidative modification of the Rubisco small subunit (SSU). These changes came with a drastic loss in pigmentation. Regarding first trifoliate leaves, +80 stimulated Rubisco activity by 33% while it disturbed neither PEPc activity nor pigmentation. Surprisingly, the enhanced Rubisco activity was associated with a slight decrease in Rubisco protein quantity, which was not coupled with the formation of carbonyl groups in Rubisco-SSU." @default.
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- W1992201149 date "2008-03-01" @default.
- W1992201149 modified "2023-09-26" @default.
- W1992201149 title "Foliar CO2 fixation in bean (Phaseolus vulgaris L.) submitted to elevated ozone: Distinct changes in Rubisco and PEPc activities in relation to pigment content" @default.
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- W1992201149 doi "https://doi.org/10.1016/j.ecoenv.2006.10.010" @default.
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