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- W1992221167 abstract "Studies of the fusion activity of respiratory syncytial virus (RSV) F protein are significantly hindered by low recombinant expression levels. While infection produces F protein levels detectable by western blot, recombinant expression produces undetectable to low levels of F protein. Identifying the obstacles that hinder recombinant F protein expression may lead to improved expression and facilitate the study of F protein function. We hypothesized that nuclear localization and/or inefficient RNA polymerase II-mediated transcription contribute to poor recombinant F protein expression. This study shows a combination of stalled nuclear export, premature polyadenylation, and low mRNA abundance all contribute to low recombinant F protein expression levels. In addition, this study provides an expression optimization strategy that results in greater F protein expression levels than observed by codon-optimization of the F protein gene, which will be useful for future studies of F protein function." @default.
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- W1992221167 date "2010-01-29" @default.
- W1992221167 modified "2023-09-27" @default.
- W1992221167 title "Recombinant respiratory syncytial virus F protein expression is hindered by inefficient nuclear export and mRNA processing" @default.
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- W1992221167 doi "https://doi.org/10.1007/s11262-010-0449-8" @default.
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