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- W1992225296 abstract "Diethyl pyrocarbonate, a compound extensively used as a nuclease inhibitor, reacts with low molecular weight RNA and forms products which are less precipitable with certain precipitating reagents than untreated RNA. This phenomenon, erroneously interpreted by Wiegers and Hilz (Biochem. Biophys. Res. Commun. 44 (1971) 513) as degradation of RNA by diethyl pyrocarbonate, is now shown to be due to increased solubility of the treated low molecular weight RNA in precipitating reagents. This conclusion is based on (a) unaltered sedimentation pattern, (b) unchanged content in dialyzable fragments and (c) identical chain length of diethyl pyrocarbonate-treated RNA as compared to the untreated control." @default.
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- W1992225296 date "1975-07-01" @default.
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- W1992225296 title "Diethyl pyrocarbonate does not degrade RNA" @default.
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- W1992225296 doi "https://doi.org/10.1016/s0006-291x(75)80174-4" @default.
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