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• W1992231285 abstract "The identification and functional characterization of pathogen-specific T cells plays a critical role in immunological research and diagnostics. In addition to the present standard technologies such as intracellular cytokine staining (ICS), enzyme-linked immunospot (ELISPOT) and peptide-major-histocompatibility-complex (MHC) multimer staining, we aimed to develop a multiplex detection assay, which provides fast in vitro functional data for both human CD4 and CD8 T cells with different antigen specificities in one sample. In this study, we have exploited the expression of CD83 on B cells to develop the cell array-based indirect T cell recognition assay (ITRA). In detail, B cells are pulsed with different pathogen peptide pools and fluorescently barcoded. Thereafter the B cells are pooled and co-cultured with autologous T cells. Subsequently each B cell population is analyzed via flow cytometry for CD83 expression, which indicates antigen-specific interaction with CD4 T cells. Moreover, we revealed donor dependent variations of cytotoxic activity of pathogen-specific CD4 T cells and CD8 T cells, evidenced by specific lysis of peptide-pulsed B cells. Taken together, ITRA is a novel antigen presenting cell (APC) array based method to analyze the presence and function of various antigen-specific T cells in one sample. It has the potential to be used in the future for epitope/antigen screening in research and for analysis of anti-tumor, anti-pathogen or autoimmune T cell responses in patient samples." @default.
• W1992231285 created "2016-06-24" @default.
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• W1992231285 date "2014-02-01" @default.
• W1992231285 modified "2023-09-27" @default.
• W1992231285 title "Multiplex and functional detection of antigen-specific human T cells by ITRA—Indirect T cell recognition assay" @default.
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• W1992231285 doi "https://doi.org/10.1016/j.jim.2013.11.027" @default.
• W1992231285 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24333463" @default.
• W1992231285 hasPublicationYear "2014" @default.
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