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• W1992255110 abstract "The aim of this work was to study selenium (Se) speciation in the potato (Solanum tuberosum L.) cultivar Desiree, enriched in Se by foliar spraying with a water solution containing 10 mg of Se/L in the form of sodium selenate. Four combinations of treatments were used: well-watered plants with and without Se foliar spraying and drought-exposed plants with and without Se foliar spraying. Water-soluble Se compounds were extracted from potato tubers by water or enzymatic hydrolysis with the enzyme protease XIV, amylase, or a combination of protease XIV and amylase. Extraction was performed using incubation at a constant temperature and stirring (37 °C at 200 rpm) or by ultrasound-assisted extraction (300 W), using different extraction times. Separation of soluble Se species (SeCys2, SeMet, SeMeSeCys, selenite, and selenate) was achieved by ion-exchange chromatography, and detection was performed by inductively coupled plasma−mass spectrometry (ICP−MS). Results showed that the concentration of selenate extracted was independent of the enzymatic extraction technique (approximately 98 ng/g for drought-exposed and 308 ng/g for well-watered potato tubers), whereas the extraction yield of SeMet changed with the protocol used (10−36%). Selenate and SeMet were the main soluble Se species (representing 51−68% of total Se) in potato tubers, regardless of the growth conditions." @default.
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• W1992255110 date "2008-09-17" @default.
• W1992255110 modified "2023-09-26" @default.
• W1992255110 title "Selenium Species in Selenium-Enriched and Drought-Exposed Potatoes" @default.
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• W1992255110 doi "https://doi.org/10.1021/jf8014969" @default.
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