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• W1992301098 abstract "ObjectiveTo determine the effect of 17β-estradiol, progesterone, medroxyprogesterone acetate, levonorgestrel, norethindrone, tibolone, and tibolone metabolites on vascular endothelial growth factor (VEGF) isoforms 121 and 165 mRNA in two breast cancer cell lines, MCF-7 (estrogen receptor rich) and T47-D (progesterone receptor rich), in vitro.DesignProspective basic research study.SettingBasic research laboratory.Patient(s)None.Intervention(s)MCF-7 and T47-D cells were cultured to 80% confluence in vitro. After 24 hours’ incubation in serum-free media, 1.0, 0.1, and 0.01 μM of 17β-estradiol, tibolone, 3α-hydroxytibolone and 3β-hydroxytibolone were added to MCF-7 cells. Progesterone, medroxyprogesterone acetate, levonorgestrel, norethindrone, and Δ4 tibolone at 1.0, 0.1, and 0.01 μM were added to T47-D cells. The cells plus steroids were incubated for a further 24 hours.Main Outcome Measure(s)Isolation and identification of VEGF isoforms 121 and 165 using semiquantitative polymerase chain reaction, gel electrophoresis, with cyclophilin as an internal control.Result(s)17β-Estradiol, tibolone, 3α-hydroxytibolone, and 3β-hydroxytibolone had no effect on VEGF mRNA in MCF-7 cells. Progesterone, medroxyprogesterone acetate, levonorgestrel, and norethindrone increased VEGF mRNA in T47-D cells. Δ4-Tibolone also increased VEGF mRNA but to a lesser extent than the progestogens.Conclusion(s)17β-estradiol, tibolone, and tibolone hydroxy-metabolites had no effect on VEGF mRNA in MCF-7 cells. Progesterone and progestins increased VEGF mRNA in T47-D breast cancer cells, but Δ4-tibolone was less effective than progestogens on this angiogenic gene in the T-47 D cells. This differential effect may be related to breast cancer growth. To determine the effect of 17β-estradiol, progesterone, medroxyprogesterone acetate, levonorgestrel, norethindrone, tibolone, and tibolone metabolites on vascular endothelial growth factor (VEGF) isoforms 121 and 165 mRNA in two breast cancer cell lines, MCF-7 (estrogen receptor rich) and T47-D (progesterone receptor rich), in vitro. Prospective basic research study. Basic research laboratory. None. MCF-7 and T47-D cells were cultured to 80% confluence in vitro. After 24 hours’ incubation in serum-free media, 1.0, 0.1, and 0.01 μM of 17β-estradiol, tibolone, 3α-hydroxytibolone and 3β-hydroxytibolone were added to MCF-7 cells. Progesterone, medroxyprogesterone acetate, levonorgestrel, norethindrone, and Δ4 tibolone at 1.0, 0.1, and 0.01 μM were added to T47-D cells. The cells plus steroids were incubated for a further 24 hours. Isolation and identification of VEGF isoforms 121 and 165 using semiquantitative polymerase chain reaction, gel electrophoresis, with cyclophilin as an internal control. 17β-Estradiol, tibolone, 3α-hydroxytibolone, and 3β-hydroxytibolone had no effect on VEGF mRNA in MCF-7 cells. Progesterone, medroxyprogesterone acetate, levonorgestrel, and norethindrone increased VEGF mRNA in T47-D cells. Δ4-Tibolone also increased VEGF mRNA but to a lesser extent than the progestogens. 17β-estradiol, tibolone, and tibolone hydroxy-metabolites had no effect on VEGF mRNA in MCF-7 cells. Progesterone and progestins increased VEGF mRNA in T47-D breast cancer cells, but Δ4-tibolone was less effective than progestogens on this angiogenic gene in the T-47 D cells. This differential effect may be related to breast cancer growth." @default.
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• W1992301098 date "2005-08-01" @default.
• W1992301098 modified "2023-09-25" @default.
• W1992301098 title "Effect of 17β-estradiol, progesterone, synthetic progestins, tibolone, and tibolone metabolites on vascular endothelial growth factor mRNA in breast cancer cells" @default.
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• W1992301098 doi "https://doi.org/10.1016/j.fertnstert.2005.01.129" @default.
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