FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992330897> ?p ?o ?g. }

• W1992330897 abstract "Nucleostemin is a newly found putative GTPase protein that binds to P53 and exists mainly in the nucleoli and at a very low level in nucleoplasm of undifferentiated embryonic stem cells (ESC) and myeloid stem cells but is not expressed in committed and terminally differentiated cells. Embryonic stem cells are pluripotent cells derived from the inner cell mass (ICM) of blastocysts. The ICM and ESC express a number of transcription factors, and their expression is used as a pluripotency marker in the ESC of many species. The present study was undertaken to identify expression of the nucleostemin gene in different developmental stages of buffalo embryos and cultured ESC. Parthenogenetic activation is a process by which an oocyte can be developed up to blastocyst without fertilization. The parthenotes were produced by following protocol. Briefly, immature oocytes were aspirated from slaughterhouse buffalo ovaries and subjected to in vitro maturation for 24 h in a CO2 incubator (5% O2, 5% CO2, 90–95% relative humidity) at 38.5°C. After 24 h of in vitro maturation, oocytes were activated by exposure to 7% ethanol for 7 min, followed by incubation with 2 mM 6-dimethyl aminopurine in CR2 medium for 3.5 h, and they were then subjected to in vitro culture. The activated embryos were cultured for 8 days in CR2 medium containing 0.6% BSA and 10% FBS to obtain different stages (immature and mature oocytes 2-, 4-,8–16-cell, morula, and blastocyst) of embryos. A total of 23 blastocysts were produced parthenogenetically, of which 5 blastocysts were used for nucleostemin expression and the rest were used for ICM isolation. The isolated ICM were subsequently cultured on mitomycin-C (10 μg mL–1) treated buffalo fetal fibroblast feeder layer in DMEM medium supplemented with 20% fetal bovine serum, 1 000 IU mL–1 of mouse leukemia inhibitory factor, 1% nonessential amino acids, 2 mM L-glutamine, and 50 μg mL–1 gentamycin. These ESC were cultured up to 5 passages. The 5 embryos of different developmental stages and a clump of ESC were used for nucleostemin expression. The total RNA was isolated and transcribed using Cell-to-cDNA-II (Ambion, Austin, TX, USA) according to manufacturer protocol. To amplify the nucleostemin gene, the PCR cycle was carried out and included heating to 94°C for 5 min, followed by 35 cycles at 94°C for 30 s, 60°C for 30 s, and 72°C for 40 s. The expressions of nucleostemin transcript were observed in all the developmental stages including immature and mature oocytes. The transcript was highly expressed in the 2-cell stage, blastocysts, and ESC, but immature oocytes and 8–16-cell stage showed lower expression. The experiment was repeated, and the same result was found. To our knowledge this is the first report in buffalo. It is concluded that the transcript was expressed in all the early stages of parthenogenetically derived buffalo embryos from immature oocytes to blastocysts and continued to be expressed in ESC. This work was funded by NAIP, C-420678075, India." @default.
• W1992330897 created "2016-06-24" @default.
• W1992330897 creator A5000391531 @default.
• W1992330897 creator A5019455636 @default.
• W1992330897 creator A5026733261 @default.
• W1992330897 creator A5026946514 @default.
• W1992330897 creator A5028800318 @default.
• W1992330897 creator A5049593255 @default.
• W1992330897 creator A5060625874 @default.
• W1992330897 creator A5066603238 @default.
• W1992330897 creator A5067534049 @default.
• W1992330897 creator A5080936109 @default.
• W1992330897 date "2011-01-01" @default.
• W1992330897 modified "2023-09-22" @default.
• W1992330897 title "311 EXPRESSION OF PLURIPOTENT MARKER NUCLEOSTEMIN IN BUFFALO (BUBALUS BUBALIS) EMBRYOS AND EMBRYONIC STEM CELLS GENERATED THROUGH PARTHENOGENETIC ACTIVATION" @default.
• W1992330897 doi "https://doi.org/10.1071/rdv23n1ab311" @default.
• W1992330897 hasPublicationYear "2011" @default.
• W1992330897 type Work @default.
• W1992330897 sameAs 1992330897 @default.
• W1992330897 citedByCount "0" @default.
• W1992330897 crossrefType "journal-article" @default.
• W1992330897 hasAuthorship W1992330897A5000391531 @default.
• W1992330897 hasAuthorship W1992330897A5019455636 @default.
• W1992330897 hasAuthorship W1992330897A5026733261 @default.
• W1992330897 hasAuthorship W1992330897A5026946514 @default.
• W1992330897 hasAuthorship W1992330897A5028800318 @default.
• W1992330897 hasAuthorship W1992330897A5049593255 @default.
• W1992330897 hasAuthorship W1992330897A5060625874 @default.
• W1992330897 hasAuthorship W1992330897A5066603238 @default.
• W1992330897 hasAuthorship W1992330897A5067534049 @default.
• W1992330897 hasAuthorship W1992330897A5080936109 @default.
• W1992330897 hasConcept C104317684 @default.
• W1992330897 hasConcept C107459253 @default.
• W1992330897 hasConcept C145103041 @default.
• W1992330897 hasConcept C153911025 @default.
• W1992330897 hasConcept C159399396 @default.
• W1992330897 hasConcept C16685009 @default.
• W1992330897 hasConcept C196843134 @default.
• W1992330897 hasConcept C207701140 @default.
• W1992330897 hasConcept C2775966511 @default.
• W1992330897 hasConcept C2776690073 @default.
• W1992330897 hasConcept C2777933648 @default.
• W1992330897 hasConcept C2778177303 @default.
• W1992330897 hasConcept C28328180 @default.
• W1992330897 hasConcept C54355233 @default.
• W1992330897 hasConcept C71924100 @default.
• W1992330897 hasConcept C86803240 @default.
• W1992330897 hasConcept C87073359 @default.
• W1992330897 hasConcept C95444343 @default.
• W1992330897 hasConceptScore W1992330897C104317684 @default.
• W1992330897 hasConceptScore W1992330897C107459253 @default.
• W1992330897 hasConceptScore W1992330897C145103041 @default.
• W1992330897 hasConceptScore W1992330897C153911025 @default.
• W1992330897 hasConceptScore W1992330897C159399396 @default.
• W1992330897 hasConceptScore W1992330897C16685009 @default.
• W1992330897 hasConceptScore W1992330897C196843134 @default.
• W1992330897 hasConceptScore W1992330897C207701140 @default.
• W1992330897 hasConceptScore W1992330897C2775966511 @default.
• W1992330897 hasConceptScore W1992330897C2776690073 @default.
• W1992330897 hasConceptScore W1992330897C2777933648 @default.
• W1992330897 hasConceptScore W1992330897C2778177303 @default.
• W1992330897 hasConceptScore W1992330897C28328180 @default.
• W1992330897 hasConceptScore W1992330897C54355233 @default.
• W1992330897 hasConceptScore W1992330897C71924100 @default.
• W1992330897 hasConceptScore W1992330897C86803240 @default.
• W1992330897 hasConceptScore W1992330897C87073359 @default.
• W1992330897 hasConceptScore W1992330897C95444343 @default.
• W1992330897 hasLocation W19923308971 @default.
• W1992330897 hasOpenAccess W1992330897 @default.
• W1992330897 hasPrimaryLocation W19923308971 @default.
• W1992330897 hasRelatedWork W1494352767 @default.
• W1992330897 hasRelatedWork W1507423443 @default.
• W1992330897 hasRelatedWork W1991072146 @default.
• W1992330897 hasRelatedWork W1992669442 @default.
• W1992330897 hasRelatedWork W1998739187 @default.
• W1992330897 hasRelatedWork W2005178452 @default.
• W1992330897 hasRelatedWork W2033192545 @default.
• W1992330897 hasRelatedWork W2052758884 @default.
• W1992330897 hasRelatedWork W2058263664 @default.
• W1992330897 hasRelatedWork W2061437770 @default.
• W1992330897 hasRelatedWork W2064278660 @default.
• W1992330897 hasRelatedWork W2065574508 @default.
• W1992330897 hasRelatedWork W2072467382 @default.
• W1992330897 hasRelatedWork W2089392593 @default.
• W1992330897 hasRelatedWork W2090776593 @default.
• W1992330897 hasRelatedWork W2183862934 @default.
• W1992330897 hasRelatedWork W2299364676 @default.
• W1992330897 hasRelatedWork W2390537189 @default.
• W1992330897 hasRelatedWork W2412040664 @default.
• W1992330897 hasRelatedWork W858944465 @default.
• W1992330897 isParatext "false" @default.
• W1992330897 isRetracted "false" @default.
• W1992330897 magId "1992330897" @default.
• W1992330897 workType "article" @default.