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• W1992357100 abstract "Oligodendrocyte mitochondria from 21-day-old Sprague-Dawley male rats were incubated with 100 nM [3H]cholesterol. It yielded [3H]pregnenolone at a rate of 2.5 +/- 0.7 and 5-[3H]pregnene-3 beta, 20 alpha-diol at a rate of 2.5 +/- 1.1 pmol per mg of protein per hr. Cultures of glial cells from 19- to 21-day-old fetuses (a mixed population of astrocytes and oligodendrocytes) were incubated for 24 hr with [3H]mevalonolactone. [3H]Cholesterol, [3H]pregnenolone, and 5-[3H]pregnene-3 beta, 20 alpha-diol were characterized in cellular extracts. The formation of the 3H-labeled steroids was increased by dibutyryl cAMP (0.2 mM) added to the culture medium. The active cholesterol side-chain cleavage mechanism, recently suggested immunohistochemically and already observed in cultures of C6 glioma cells, reinforces the concept of neurosteroids applied to delta 5-3 beta-hydroxysteroids previously isolated from brain." @default.
• W1992357100 created "2016-06-24" @default.
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• W1992357100 date "1987-12-01" @default.
• W1992357100 modified "2023-10-18" @default.
• W1992357100 title "Neurosteroids: oligodendrocyte mitochondria convert cholesterol to pregnenolone." @default.
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• W1992357100 doi "https://doi.org/10.1073/pnas.84.23.8215" @default.
• W1992357100 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/299512" @default.
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