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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992372005> ?p ?o ?g. }

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• W1992372005 abstract "Sensitive and specific enzyme-linked immunosorbent assays were developed to detect Clostridium botulinum neurotoxin serotypes A (BoNT A) and B (BoNT B) in assay buffer and human serum. The assay is based upon affinity-purified horse polyclonal antibodies directed against the ∼50 kDa C-fragments of each toxin. Standard curves were linear over the range of 0.1–10 ng/mL. Detection was possible at 0.2 ng/mL (20 pg/well) and accurate quantitation at 0.5 ng/mL (50 pg/well) in assay buffer and 10% human serum. Variations between triplicates was typically 5–10%. Less than 1% cross reactivity occurred between other serotypes when each assay was performed against serotypes A, B and E. When tested against toxins complexed to their associated nontoxic proteins, interference was absent (BoNT B) or <25% (BoNT A). These assays demonstrate sensitivity close to that of the mouse bioassay without the use of animals and in a much simpler format than other reported assays of similar sensitivity." @default.
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• W1992372005 date "2000-03-01" @default.
• W1992372005 modified "2023-09-25" @default.
• W1992372005 title "Development of sensitive colorimetric capture elisas for Clostridium botulinum neurotoxin serotypes A and B" @default.
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• W1992372005 doi "https://doi.org/10.1016/s0041-0101(99)00168-3" @default.
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