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- W1992391204 abstract "HydEn-seq, a new sequencing method that maps the distribution of ribonucleotides misincorporated by low-fidelity DNA polymerases in budding yeast, reveals unexpected strand-specific replication patterns in both nuclear and mitochondrial genomes. Ribonucleotides are frequently incorporated into DNA during replication in eukaryotes. Here we map genome-wide distribution of these ribonucleotides as markers of replication enzymology in budding yeast, using a new 5′ DNA end–mapping method, hydrolytic end sequencing (HydEn-seq). HydEn-seq of DNA from ribonucleotide excision repair–deficient strains reveals replicase- and strand-specific patterns of ribonucleotides in the nuclear genome. These patterns support the roles of DNA polymerases α and δ in lagging-strand replication and of DNA polymerase ɛ in leading-strand replication. They identify replication origins, termination zones and variations in ribonucleotide incorporation frequency across the genome that exceed three orders of magnitude. HydEn-seq also reveals strand-specific 5′ DNA ends at mitochondrial replication origins, thus suggesting unidirectional replication of a circular genome. Given the conservation of enzymes that incorporate and process ribonucleotides in DNA, HydEn-seq can be used to track replication enzymology in other organisms." @default.
- W1992391204 created "2016-06-24" @default.
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- W1992391204 date "2015-01-26" @default.
- W1992391204 modified "2023-10-01" @default.
- W1992391204 title "Tracking replication enzymology in vivo by genome-wide mapping of ribonucleotide incorporation" @default.
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- W1992391204 doi "https://doi.org/10.1038/nsmb.2957" @default.
- W1992391204 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4351163" @default.
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