FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992471594> ?p ?o ?g. }

• W1992471594 endingPage "S73" @default.
• W1992471594 startingPage "S65" @default.
• W1992471594 abstract "The bacterial enzyme D-xylose isomerase (XI) catalyses the conversion of D-xylose to D-xylulose. Each subunit of the homotetrameric protein contains a bimetallic active centre requiring divalent metal ions such as Mg2+, Mn2+ or Co2+ for catalytic activity. We report here on XI in which the metal binding site 1 is specifically loaded with EPR active Mn2+, while binding site 2 is occupied by Co2+ or Cd2+, rendering a catalytically active or inactive species respectively. The Q-band (34 GHz) EPR spectra of these mixed-metal samples (Co2+/Mn2+ and Cd2+/Mn2+ XI) show a clear influence of the metal in site 2 on the Mn2+ EPR parameters. Likewise, a systematic increase of the zero field splitting parameters (zfs) of Mn2+ in site 1 upon incubation with the inhibitor xylitol or substrates for both mixed-metal samples is found. For Co2+/Mn2+ XI complexed with substrate, a drastic line broadening of the central -1/2 <--> +1/2 transition is observed in Q-band EPR, which was not amenable to analysis so far. By means of a multi-frequency approach at frequencies beyond Q-band, the relevant zfs parameters were derived from spectral simulations of EPR spectra measured at 94, 285 and 670 GHz. It is shown that parallel to the increase of the D-value its distribution also grows considerably in going from free Co2+/Mn2+ XI to the species complexed with inhibitor or substrate. For XI with bound substrate, D-values in the range of 70-90 mT and a distribution of about 30 mT were found from simulation trials. The large distribution in zfs values is thought to be correlated to the structural disorder induced by the shift of the metal ion of site 2 into a location necessary for the isomerisation reaction. The results are discussed with respect to high-resolution crystal data." @default.
• W1992471594 created "2016-06-24" @default.
• W1992471594 creator A5002581586 @default.
• W1992471594 creator A5031720787 @default.
• W1992471594 creator A5044326170 @default.
• W1992471594 creator A5064094423 @default.
• W1992471594 creator A5069610487 @default.
• W1992471594 date "2005-01-01" @default.
• W1992471594 modified "2023-10-12" @default.
• W1992471594 title "Multi-frequency high-field EPR studies on metal-substituted xylose isomerase" @default.
• W1992471594 cites W1500330894 @default.
• W1992471594 cites W1965194719 @default.
• W1992471594 cites W1968185653 @default.
• W1992471594 cites W1972065201 @default.
• W1992471594 cites W1973691085 @default.
• W1992471594 cites W1974821910 @default.
• W1992471594 cites W1976467474 @default.
• W1992471594 cites W1977750999 @default.
• W1992471594 cites W1981070705 @default.
• W1992471594 cites W1986842116 @default.
• W1992471594 cites W1987671987 @default.
• W1992471594 cites W1988231282 @default.
• W1992471594 cites W1990835297 @default.
• W1992471594 cites W1993684667 @default.
• W1992471594 cites W1996565778 @default.
• W1992471594 cites W1996680929 @default.
• W1992471594 cites W1998959523 @default.
• W1992471594 cites W2010191623 @default.
• W1992471594 cites W2013425831 @default.
• W1992471594 cites W2019002763 @default.
• W1992471594 cites W2024095356 @default.
• W1992471594 cites W2025152052 @default.
• W1992471594 cites W2045832806 @default.
• W1992471594 cites W2050529900 @default.
• W1992471594 cites W2054683113 @default.
• W1992471594 cites W2062497256 @default.
• W1992471594 cites W2065825261 @default.
• W1992471594 cites W2066916400 @default.
• W1992471594 cites W2070710792 @default.
• W1992471594 cites W2070795446 @default.
• W1992471594 cites W2073037568 @default.
• W1992471594 cites W2082632472 @default.
• W1992471594 cites W2086609868 @default.
• W1992471594 cites W2112554771 @default.
• W1992471594 cites W2167590372 @default.
• W1992471594 cites W2288091732 @default.
• W1992471594 cites W2441717395 @default.
• W1992471594 cites W4255181400 @default.
• W1992471594 cites W42695881 @default.
• W1992471594 cites W94163313 @default.
• W1992471594 doi "https://doi.org/10.1002/mrc.1691" @default.
• W1992471594 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16235215" @default.
• W1992471594 hasPublicationYear "2005" @default.
• W1992471594 type Work @default.
• W1992471594 sameAs 1992471594 @default.
• W1992471594 citedByCount "6" @default.
• W1992471594 countsByYear W19924715942017 @default.
• W1992471594 crossrefType "journal-article" @default.
• W1992471594 hasAuthorship W1992471594A5002581586 @default.
• W1992471594 hasAuthorship W1992471594A5031720787 @default.
• W1992471594 hasAuthorship W1992471594A5044326170 @default.
• W1992471594 hasAuthorship W1992471594A5064094423 @default.
• W1992471594 hasAuthorship W1992471594A5069610487 @default.
• W1992471594 hasConcept C111368507 @default.
• W1992471594 hasConcept C113196181 @default.
• W1992471594 hasConcept C121332964 @default.
• W1992471594 hasConcept C127313418 @default.
• W1992471594 hasConcept C1276947 @default.
• W1992471594 hasConcept C178790620 @default.
• W1992471594 hasConcept C181199279 @default.
• W1992471594 hasConcept C185592680 @default.
• W1992471594 hasConcept C187961010 @default.
• W1992471594 hasConcept C2777289219 @default.
• W1992471594 hasConcept C41183919 @default.
• W1992471594 hasConcept C43411465 @default.
• W1992471594 hasConcept C46141821 @default.
• W1992471594 hasConcept C4839761 @default.
• W1992471594 hasConcept C544153396 @default.
• W1992471594 hasConcept C71240020 @default.
• W1992471594 hasConcept C8010536 @default.
• W1992471594 hasConceptScore W1992471594C111368507 @default.
• W1992471594 hasConceptScore W1992471594C113196181 @default.
• W1992471594 hasConceptScore W1992471594C121332964 @default.
• W1992471594 hasConceptScore W1992471594C127313418 @default.
• W1992471594 hasConceptScore W1992471594C1276947 @default.
• W1992471594 hasConceptScore W1992471594C178790620 @default.
• W1992471594 hasConceptScore W1992471594C181199279 @default.
• W1992471594 hasConceptScore W1992471594C185592680 @default.
• W1992471594 hasConceptScore W1992471594C187961010 @default.
• W1992471594 hasConceptScore W1992471594C2777289219 @default.
• W1992471594 hasConceptScore W1992471594C41183919 @default.
• W1992471594 hasConceptScore W1992471594C43411465 @default.
• W1992471594 hasConceptScore W1992471594C46141821 @default.
• W1992471594 hasConceptScore W1992471594C4839761 @default.
• W1992471594 hasConceptScore W1992471594C544153396 @default.
• W1992471594 hasConceptScore W1992471594C71240020 @default.
• W1992471594 hasConceptScore W1992471594C8010536 @default.
• W1992471594 hasIssue "S1" @default.

• next