FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992507941> ?p ?o ?g. }

• W1992507941 endingPage "1158" @default.
• W1992507941 startingPage "1152" @default.
• W1992507941 abstract "Pyrrole-imidazole (PI) polyamides are a novel group of gene-silencing compounds, which bind to a minor groove of double stranded (ds)DNA in a sequence-specific manner. To explore the RNA binding properties of PI polyamides targeting rat transforming growth factor-β1 (TGF-β1 Polyamide) and influenza A virus (PA polyamide), we designed dsRNAs with an identical sequence to the target DNA and analyzed RNA binding properties of the polyamide. Biacore assay showed fast binding of TGF-β1 Polyamide to the dsRNA, whereas mismatch polyamide did not bind to the dsRNA. Dissociation equilibrium constant (KD) value was 6.7×10−7 of the target dsRNA. These results indicate that PI polyamide could bind to RNA with a 2 log lower binding affinity than its DNA-binding affinity. We designed a PI polyamide targeting the panhandle stem region of influenza A virus. KD value of the PI polyamide to dsRNA targeting influenza A virus was 4.6×10−7. Gel-shift assay showed that TGF-β1 and PA polyamides bound to the appropriate dsDNA, whereas these PI polyamides did not show obvious gel-shift with the appropriate dsRNA. Structural modeling suggests that PI polyamide binds to the appropriate B-form dsDNA in the minor groove, whereas it does not fit in the minor groove to dsRNA. Thus PI polyamides have a lower binding affinity with target dsRNA than they do with dsDNA. The distinct binding properties of PI polyamides to dsRNA and dsDNA may be associated with differences of secondary structure and chemical binding properties between target RNA and DNA." @default.
• W1992507941 created "2016-06-24" @default.
• W1992507941 creator A5017785312 @default.
• W1992507941 creator A5021979476 @default.
• W1992507941 creator A5032412570 @default.
• W1992507941 creator A5034851349 @default.
• W1992507941 creator A5036716509 @default.
• W1992507941 creator A5038984650 @default.
• W1992507941 creator A5054313221 @default.
• W1992507941 creator A5066291252 @default.
• W1992507941 creator A5089578680 @default.
• W1992507941 date "2013-01-01" @default.
• W1992507941 modified "2023-09-24" @default.
• W1992507941 title "RNA Binding Properties of Novel Gene Silencing Pyrrole-Imidazole Polyamides" @default.
• W1992507941 cites W1963834745 @default.
• W1992507941 cites W2004945910 @default.
• W1992507941 cites W2017764099 @default.
• W1992507941 cites W2017975664 @default.
• W1992507941 cites W2018996043 @default.
• W1992507941 cites W2023370195 @default.
• W1992507941 cites W2029842408 @default.
• W1992507941 cites W2038886788 @default.
• W1992507941 cites W2038985545 @default.
• W1992507941 cites W2043548965 @default.
• W1992507941 cites W2052021526 @default.
• W1992507941 cites W2056104022 @default.
• W1992507941 cites W2056882517 @default.
• W1992507941 cites W2064734340 @default.
• W1992507941 cites W2085820867 @default.
• W1992507941 cites W2106367530 @default.
• W1992507941 cites W2148576714 @default.
• W1992507941 cites W2158738287 @default.
• W1992507941 doi "https://doi.org/10.1248/bpb.b13-00135" @default.
• W1992507941 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23628892" @default.
• W1992507941 hasPublicationYear "2013" @default.
• W1992507941 type Work @default.
• W1992507941 sameAs 1992507941 @default.
• W1992507941 citedByCount "17" @default.
• W1992507941 countsByYear W19925079412014 @default.
• W1992507941 countsByYear W19925079412015 @default.
• W1992507941 countsByYear W19925079412016 @default.
• W1992507941 countsByYear W19925079412019 @default.
• W1992507941 countsByYear W19925079412020 @default.
• W1992507941 countsByYear W19925079412021 @default.
• W1992507941 countsByYear W19925079412022 @default.
• W1992507941 countsByYear W19925079412023 @default.
• W1992507941 crossrefType "journal-article" @default.
• W1992507941 hasAuthorship W1992507941A5017785312 @default.
• W1992507941 hasAuthorship W1992507941A5021979476 @default.
• W1992507941 hasAuthorship W1992507941A5032412570 @default.
• W1992507941 hasAuthorship W1992507941A5034851349 @default.
• W1992507941 hasAuthorship W1992507941A5036716509 @default.
• W1992507941 hasAuthorship W1992507941A5038984650 @default.
• W1992507941 hasAuthorship W1992507941A5054313221 @default.
• W1992507941 hasAuthorship W1992507941A5066291252 @default.
• W1992507941 hasAuthorship W1992507941A5089578680 @default.
• W1992507941 hasBestOaLocation W19925079411 @default.
• W1992507941 hasConcept C104317684 @default.
• W1992507941 hasConcept C107824862 @default.
• W1992507941 hasConcept C153911025 @default.
• W1992507941 hasConcept C166703698 @default.
• W1992507941 hasConcept C185592680 @default.
• W1992507941 hasConcept C188027245 @default.
• W1992507941 hasConcept C190399342 @default.
• W1992507941 hasConcept C2191507 @default.
• W1992507941 hasConcept C552990157 @default.
• W1992507941 hasConcept C55493867 @default.
• W1992507941 hasConcept C67705224 @default.
• W1992507941 hasConcept C71240020 @default.
• W1992507941 hasConcept C86803240 @default.
• W1992507941 hasConceptScore W1992507941C104317684 @default.
• W1992507941 hasConceptScore W1992507941C107824862 @default.
• W1992507941 hasConceptScore W1992507941C153911025 @default.
• W1992507941 hasConceptScore W1992507941C166703698 @default.
• W1992507941 hasConceptScore W1992507941C185592680 @default.
• W1992507941 hasConceptScore W1992507941C188027245 @default.
• W1992507941 hasConceptScore W1992507941C190399342 @default.
• W1992507941 hasConceptScore W1992507941C2191507 @default.
• W1992507941 hasConceptScore W1992507941C552990157 @default.
• W1992507941 hasConceptScore W1992507941C55493867 @default.
• W1992507941 hasConceptScore W1992507941C67705224 @default.
• W1992507941 hasConceptScore W1992507941C71240020 @default.
• W1992507941 hasConceptScore W1992507941C86803240 @default.
• W1992507941 hasIssue "7" @default.
• W1992507941 hasLocation W19925079411 @default.
• W1992507941 hasLocation W19925079412 @default.
• W1992507941 hasOpenAccess W1992507941 @default.
• W1992507941 hasPrimaryLocation W19925079411 @default.
• W1992507941 hasRelatedWork W1966243747 @default.
• W1992507941 hasRelatedWork W1976736675 @default.
• W1992507941 hasRelatedWork W1985755098 @default.
• W1992507941 hasRelatedWork W1992579553 @default.
• W1992507941 hasRelatedWork W2002473487 @default.
• W1992507941 hasRelatedWork W2034409080 @default.
• W1992507941 hasRelatedWork W2034785326 @default.
• W1992507941 hasRelatedWork W2080586954 @default.
• W1992507941 hasRelatedWork W2122550126 @default.
• W1992507941 hasRelatedWork W2160783980 @default.

• next