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- W1992510139 abstract "Abstract The activities of the various phosphatases on the plasma membrane of normal and leukemic mouse thymocytes were delineated using the specific inhibitors, α, β-methylene ADP, which inhibits 5′-nucleotidase while not affecting nonspecific phosphatases, (−)-p-bromotetramisole, which inhibits nonspecific alkaline phosphatase, and the competitive substrate p-nitrophenylphosphate. The Km for AMP and the Ki for the inhibition of AMPase by α, β-methylene ADP at pH 7.5 were 13 and 38 μM, respectively. The activity also shows substrate inhibition at this pH. 30% of the AMPase activity could not be inhibited, and an Eadie-Hofstee plot shows mixed inhibition. Inability of the combined use of α, β-methylene ADP and (−)-p-bromotetramisole to totally suppress activity indicates the presence of a third phosphatase enzyme. Subsequent analysis at various pH values indicates that this third enzyme has an activity peak in the acid range. Using β-glycerophosphate as a substrate, 81% of the activity at pH 9.5 is due to alkaline phosphatase and none is due to 5′-nucleotidase. Likewise virtually none of the β-glycerophosphatase activity at pH 7.5 is due to alkaline phosphatase. With both Swiss albino and AKR mice, activity of 5′-nucleotidase increased markedly in adult male mice when compared to younger immature animals whereas alkaline phosphatase showed little change. In leukemic AKR mice, 5′-nucleotidase activity was sharply lower in the cells of thymomas when compared to nonleukemic AKR mice of comparable age. Alkaline phosphatase activity in the thymus of leukemic AKR mice was either increased markedly or remained unchanged. Thus the specificity of three distinct phosphatase activities in thymocytes are clearly defined and monitored during developmental and pathological alterations of the cell: (1) 5′-nucleotidase, (2) alkaline phosphatase, and (3) a third phosphatase with a lower pH optimum." @default.
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- W1992510139 date "1983-04-01" @default.
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- W1992510139 title "Delineation of membrane-bound phosphatase activities in normal and leukemic thymocytes" @default.
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- W1992510139 doi "https://doi.org/10.1016/0304-4165(83)90349-5" @default.
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