Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992588894> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1992588894 abstract "The 876-aa protein AreA regulates the expression of numerous genes involved in nitrogen metabolism in Aspergillus nidulans, and interacts with GATA sequences upstream of the relevant genes. We have carried out limited proteolysis of the C-terminal domain of the AreA protein in order to identify possible structural domains within the protein. A stable 156-amino-acid fragment was identified that contained the zinc finger region, and this sequence was cloned and expressed in E. coli. Fluorescence spectroscopy of the purified protein showed that the proteolytic domain was folded and could be denatured by high concentrations of urea (approximately 4 M), exhibiting a sharp transition. Fluorescence spectroscopy was also used to monitor binding to a DNA duplex containing the AreA recognition site, demonstrating tight binding of the domain to its DNA recognition sequence. The DNA binding affinity of the domain is comparable with that of the native AreA protein and much higher than that of the minimal zinc finger region of AreA." @default.
- W1992588894 created "2016-06-24" @default.
- W1992588894 creator A5039465575 @default.
- W1992588894 creator A5046931735 @default.
- W1992588894 creator A5059744923 @default.
- W1992588894 creator A5082857430 @default.
- W1992588894 date "2003-05-01" @default.
- W1992588894 modified "2023-09-26" @default.
- W1992588894 title "Structural and functional characterisation of the DNA binding domain of the Aspergillus nidulans gene regulatory protein AreA" @default.
- W1992588894 cites W122783581 @default.
- W1992588894 cites W13114793 @default.
- W1992588894 cites W1530639541 @default.
- W1992588894 cites W1890698840 @default.
- W1992588894 cites W2028948668 @default.
- W1992588894 cites W2039864965 @default.
- W1992588894 cites W2053603508 @default.
- W1992588894 cites W2085927701 @default.
- W1992588894 cites W2102892077 @default.
- W1992588894 cites W2106269300 @default.
- W1992588894 cites W2116608195 @default.
- W1992588894 cites W2117163537 @default.
- W1992588894 cites W2117736073 @default.
- W1992588894 cites W2170064419 @default.
- W1992588894 cites W2209090764 @default.
- W1992588894 cites W234270905 @default.
- W1992588894 cites W2488644218 @default.
- W1992588894 cites W658325284 @default.
- W1992588894 cites W2416996549 @default.
- W1992588894 doi "https://doi.org/10.1016/s1570-9639(03)00109-2" @default.
- W1992588894 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12758150" @default.
- W1992588894 hasPublicationYear "2003" @default.
- W1992588894 type Work @default.
- W1992588894 sameAs 1992588894 @default.
- W1992588894 citedByCount "2" @default.
- W1992588894 countsByYear W19925888942016 @default.
- W1992588894 crossrefType "journal-article" @default.
- W1992588894 hasAuthorship W1992588894A5039465575 @default.
- W1992588894 hasAuthorship W1992588894A5046931735 @default.
- W1992588894 hasAuthorship W1992588894A5059744923 @default.
- W1992588894 hasAuthorship W1992588894A5082857430 @default.
- W1992588894 hasConcept C101762097 @default.
- W1992588894 hasConcept C104317684 @default.
- W1992588894 hasConcept C107824862 @default.
- W1992588894 hasConcept C143065580 @default.
- W1992588894 hasConcept C150194340 @default.
- W1992588894 hasConcept C153911025 @default.
- W1992588894 hasConcept C181199279 @default.
- W1992588894 hasConcept C186055190 @default.
- W1992588894 hasConcept C24284526 @default.
- W1992588894 hasConcept C25166345 @default.
- W1992588894 hasConcept C2776923806 @default.
- W1992588894 hasConcept C2781307694 @default.
- W1992588894 hasConcept C33987129 @default.
- W1992588894 hasConcept C3662595 @default.
- W1992588894 hasConcept C5179208 @default.
- W1992588894 hasConcept C552990157 @default.
- W1992588894 hasConcept C55493867 @default.
- W1992588894 hasConcept C86339819 @default.
- W1992588894 hasConcept C86803240 @default.
- W1992588894 hasConcept C94966510 @default.
- W1992588894 hasConceptScore W1992588894C101762097 @default.
- W1992588894 hasConceptScore W1992588894C104317684 @default.
- W1992588894 hasConceptScore W1992588894C107824862 @default.
- W1992588894 hasConceptScore W1992588894C143065580 @default.
- W1992588894 hasConceptScore W1992588894C150194340 @default.
- W1992588894 hasConceptScore W1992588894C153911025 @default.
- W1992588894 hasConceptScore W1992588894C181199279 @default.
- W1992588894 hasConceptScore W1992588894C186055190 @default.
- W1992588894 hasConceptScore W1992588894C24284526 @default.
- W1992588894 hasConceptScore W1992588894C25166345 @default.
- W1992588894 hasConceptScore W1992588894C2776923806 @default.
- W1992588894 hasConceptScore W1992588894C2781307694 @default.
- W1992588894 hasConceptScore W1992588894C33987129 @default.
- W1992588894 hasConceptScore W1992588894C3662595 @default.
- W1992588894 hasConceptScore W1992588894C5179208 @default.
- W1992588894 hasConceptScore W1992588894C552990157 @default.
- W1992588894 hasConceptScore W1992588894C55493867 @default.
- W1992588894 hasConceptScore W1992588894C86339819 @default.
- W1992588894 hasConceptScore W1992588894C86803240 @default.
- W1992588894 hasConceptScore W1992588894C94966510 @default.
- W1992588894 hasLocation W19925888941 @default.
- W1992588894 hasLocation W19925888942 @default.
- W1992588894 hasOpenAccess W1992588894 @default.
- W1992588894 hasPrimaryLocation W19925888941 @default.
- W1992588894 hasRelatedWork W1991272306 @default.
- W1992588894 hasRelatedWork W1992588894 @default.
- W1992588894 hasRelatedWork W2018715590 @default.
- W1992588894 hasRelatedWork W2049414721 @default.
- W1992588894 hasRelatedWork W2051011427 @default.
- W1992588894 hasRelatedWork W2070672965 @default.
- W1992588894 hasRelatedWork W2118598814 @default.
- W1992588894 hasRelatedWork W2139734346 @default.
- W1992588894 hasRelatedWork W2159770227 @default.
- W1992588894 hasRelatedWork W2790488705 @default.
- W1992588894 isParatext "false" @default.
- W1992588894 isRetracted "false" @default.
- W1992588894 magId "1992588894" @default.
- W1992588894 workType "article" @default.