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- W1992598353 abstract "A 96-well microtiter enzyme-linked immunosorbent assay was developed to assay the activity of the cytoplasmic domain of trkA tyrosine kinase. The assay involves immobilization of phospholipase C- gamma/glutathione S-transferase fusion protein on a microtiter plate, addition of the kinase reaction mixture, and detection by an antibody to phosphotyrosine followed by an alkaline phosphatase-conjugated second antibody. The substrate used in this system, phospholipase C-gamma, is one of several biologically important substrates for the phosphorylation reaction of receptor-linked tyrosine kinases. The assay was then used to characterize kinase inhibitory activities of various small molecules including analogs of K-252a." @default.
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- W1992598353 date "1996-04-01" @default.
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- W1992598353 title "Enzyme-Linked Immunosorbent Assay for trkA Tyrosine Kinase Activity" @default.
- W1992598353 doi "https://doi.org/10.1006/abio.1996.0130" @default.
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