FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992632443> ?p ?o ?g. }

• W1992632443 endingPage "243" @default.
• W1992632443 startingPage "235" @default.
• W1992632443 abstract "Abstract As the market requirements for adenovirus vectors (AdV) increase, the maximisation of the virus titer per culture volume per unit time is a key requirement. However, despite the fact that 293 cells can grow up to 8 × 10 6 cell/mL in simple batch mode operations, for optimal AdV infection a maximum cell density of 1 × 10 6 cell/mL at infection time has usually been utilized due to the so called “cell density effect”. In addition, AdV titer appears to be dependent upon cell cycle phase at the time of infection. To evaluate the dependence of AdV production upon cell cycle phase, 293 cells were chemically synchronised at each phase of the cell cycle; a 2.6‐fold increase on AdV cell specific titer was obtained when the percentage of cells at the S phase of the cell cycle was increased from 36 to 47%; a mathematical equation was used to relate AdV cell specific productivities with cell synchronisation at the S phase using this data. To avoid the use of chemical inhibitors, a temperature shift strategy was also used for synchronisation at the S phase. S phase synchronisation was obtained by decreasing the culture temperature to 31°C during 67 h and restoring it to 37°C during 72 h. By using this strategy we were able to synchronise 57% of the population in the S phase of the cell cycle obtaining an increase of 7.3‐fold on AdV cell specific titer after infection. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009" @default.
• W1992632443 created "2016-06-24" @default.
• W1992632443 creator A5006207038 @default.
• W1992632443 creator A5021060711 @default.
• W1992632443 creator A5036398869 @default.
• W1992632443 creator A5041231390 @default.
• W1992632443 creator A5053115973 @default.
• W1992632443 creator A5058977336 @default.
• W1992632443 creator A5066980482 @default.
• W1992632443 date "2009-01-01" @default.
• W1992632443 modified "2023-10-10" @default.
• W1992632443 title "293 cell cycle synchronisation adenovirus vector production" @default.
• W1992632443 cites W143591051 @default.
• W1992632443 cites W147246515 @default.
• W1992632443 cites W1485208409 @default.
• W1992632443 cites W1965154703 @default.
• W1992632443 cites W1974007211 @default.
• W1992632443 cites W1977504181 @default.
• W1992632443 cites W1995022357 @default.
• W1992632443 cites W1995253852 @default.
• W1992632443 cites W2000650519 @default.
• W1992632443 cites W2008071343 @default.
• W1992632443 cites W2009581830 @default.
• W1992632443 cites W2020405271 @default.
• W1992632443 cites W2043228528 @default.
• W1992632443 cites W2053817512 @default.
• W1992632443 cites W2055759808 @default.
• W1992632443 cites W2062656476 @default.
• W1992632443 cites W2063708094 @default.
• W1992632443 cites W2064028836 @default.
• W1992632443 cites W2072108736 @default.
• W1992632443 cites W2072811597 @default.
• W1992632443 cites W2080381436 @default.
• W1992632443 cites W2084976117 @default.
• W1992632443 cites W2086254928 @default.
• W1992632443 cites W2092824967 @default.
• W1992632443 cites W2100885424 @default.
• W1992632443 cites W2104693664 @default.
• W1992632443 cites W2114445152 @default.
• W1992632443 cites W2122777324 @default.
• W1992632443 cites W2138858430 @default.
• W1992632443 cites W2160724997 @default.
• W1992632443 cites W2326539204 @default.
• W1992632443 cites W4229617316 @default.
• W1992632443 doi "https://doi.org/10.1002/btpr.64" @default.
• W1992632443 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19199366" @default.
• W1992632443 hasPublicationYear "2009" @default.
• W1992632443 type Work @default.
• W1992632443 sameAs 1992632443 @default.
• W1992632443 citedByCount "23" @default.
• W1992632443 countsByYear W19926324432012 @default.
• W1992632443 countsByYear W19926324432014 @default.
• W1992632443 countsByYear W19926324432015 @default.
• W1992632443 countsByYear W19926324432016 @default.
• W1992632443 countsByYear W19926324432017 @default.
• W1992632443 countsByYear W19926324432020 @default.
• W1992632443 countsByYear W19926324432021 @default.
• W1992632443 countsByYear W19926324432023 @default.
• W1992632443 crossrefType "journal-article" @default.
• W1992632443 hasAuthorship W1992632443A5006207038 @default.
• W1992632443 hasAuthorship W1992632443A5021060711 @default.
• W1992632443 hasAuthorship W1992632443A5036398869 @default.
• W1992632443 hasAuthorship W1992632443A5041231390 @default.
• W1992632443 hasAuthorship W1992632443A5053115973 @default.
• W1992632443 hasAuthorship W1992632443A5058977336 @default.
• W1992632443 hasAuthorship W1992632443A5066980482 @default.
• W1992632443 hasBestOaLocation W19926324431 @default.
• W1992632443 hasConcept C1491633281 @default.
• W1992632443 hasConcept C159047783 @default.
• W1992632443 hasConcept C178790620 @default.
• W1992632443 hasConcept C185592680 @default.
• W1992632443 hasConcept C2522874641 @default.
• W1992632443 hasConcept C2908647359 @default.
• W1992632443 hasConcept C29537977 @default.
• W1992632443 hasConcept C32611913 @default.
• W1992632443 hasConcept C44280652 @default.
• W1992632443 hasConcept C48900799 @default.
• W1992632443 hasConcept C54355233 @default.
• W1992632443 hasConcept C55493867 @default.
• W1992632443 hasConcept C62112901 @default.
• W1992632443 hasConcept C71924100 @default.
• W1992632443 hasConcept C81885089 @default.
• W1992632443 hasConcept C86803240 @default.
• W1992632443 hasConcept C95444343 @default.
• W1992632443 hasConcept C99454951 @default.
• W1992632443 hasConceptScore W1992632443C1491633281 @default.
• W1992632443 hasConceptScore W1992632443C159047783 @default.
• W1992632443 hasConceptScore W1992632443C178790620 @default.
• W1992632443 hasConceptScore W1992632443C185592680 @default.
• W1992632443 hasConceptScore W1992632443C2522874641 @default.
• W1992632443 hasConceptScore W1992632443C2908647359 @default.
• W1992632443 hasConceptScore W1992632443C29537977 @default.
• W1992632443 hasConceptScore W1992632443C32611913 @default.
• W1992632443 hasConceptScore W1992632443C44280652 @default.
• W1992632443 hasConceptScore W1992632443C48900799 @default.
• W1992632443 hasConceptScore W1992632443C54355233 @default.
• W1992632443 hasConceptScore W1992632443C55493867 @default.
• W1992632443 hasConceptScore W1992632443C62112901 @default.

• next