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• W1992639306 endingPage "161" @default.
• W1992639306 startingPage "147" @default.
• W1992639306 abstract "Pituitary adenylyl cyclase-activating polypeptide (PACAP) is a neuropeptide originally isolated from the hypothalamus, named for its high potency in stimulating adenylyl cyclase in pituitary cells. PACAP acts through the specific receptor PAC1-R to modulate the action of neurotransmitters, and additionally, to regulate cell viability via autocrine/intracrine mechanisms. Evidence has now been obtained that PACAP and multiple splice variants of PAC1-R are expressed in the rat cochlea. mRNA for PACAP precursor protein is found by reverse transcription-polymerase chain reaction (RT-PCR) in microdissected cochlear lateral wall, organ of Corti, and spiral ganglion subfractions. A specific pattern of expression of mRNA for PAC1-R splice variants, which mediate the response to PACAP, has been revealed by RT-PCR and cloning for the cochlear subfractions. Transcript for the short form of PAC1-R is found in all three subfractions. Four additional splice variants -- hop1, hop2, hip, and a novel hop1 splice variant -- are expressed in the lateral wall. For the amino terminus splice region of PAC1-R, a new splice variant has been detected in the organ of Corti, representing a deletion of the first 7 of 21 amino acids detected in the PAC1-R very-short sequence. Overall, from message determinations in cochlear subfractions, there are five PAC1-R splice variants in the lateral wall, two in the organ of Corti and one in the spiral ganglion, indicating multiple possible responses to PACAP and/or mechanisms to modulate the response to PACAP in the cochlea. The variety of PAC1-R splice variants expressed may reflect the diversity in cell function between subfractions that is modulated by PACAP. The neuropeptide and its specific receptor have been immunolocalized in the lateral wall, the source of the largest number of cochlear PAC1-R splice variants. The receptor was targeted by primary antibodies which would elicit immunoreactivity for all splice variants of PAC1-R detected with RT-PCR, and evidence has been obtained with Western blot analysis suggesting that PAC1-R is glycosylated in vivo. Within the lateral wall, PACAP and PAC1-R were immunolocalized primarily to the stria vascularis, with immunoreactivity for both neuropeptide and receptor increasing from the basal to apical cochlear turns. Within the stria, PACAP immunoreactivity was localized to the basolateral extensions of marginal cells, while PAC1-R was clearly associated with tight junctions between the marginal cells close to the endolymphatic compartment. In addition, evidence was obtained that PAC1-R was associated with endothelial cells of the capillaries in the stria vascularis. The large number of splice variants expressed, coupled to the specificity in linkage between PAC1-R splice variants and G-protein-coupled second messenger pathways, could provide a mechanism to closely modulate tight junction integrity in the stria vascularis, impacting the endolymphatic potential." @default.
• W1992639306 created "2016-06-24" @default.
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• W1992639306 date "2006-01-01" @default.
• W1992639306 modified "2023-10-16" @default.
• W1992639306 title "Pituitary Adenylyl Cyclase-Activating Polypeptide (PACAP) and its receptor (PAC1-R) in the cochlea: Evidence for specific transcript expression of PAC1-R splice variants in rat microdissected cochlear subfractions" @default.
• W1992639306 cites W1555960510 @default.
• W1992639306 cites W1604752267 @default.
• W1992639306 cites W1963724905 @default.
• W1992639306 cites W1964628076 @default.
• W1992639306 cites W1964761526 @default.
• W1992639306 cites W1966203348 @default.
• W1992639306 cites W1966707756 @default.
• W1992639306 cites W1967256984 @default.
• W1992639306 cites W1975029836 @default.
• W1992639306 cites W1976882461 @default.
• W1992639306 cites W1979067492 @default.
• W1992639306 cites W1981423842 @default.
• W1992639306 cites W1981775121 @default.
• W1992639306 cites W1982745613 @default.
• W1992639306 cites W1990422815 @default.
• W1992639306 cites W1998010389 @default.
• W1992639306 cites W2000580750 @default.
• W1992639306 cites W2008453186 @default.
• W1992639306 cites W2011893935 @default.
• W1992639306 cites W2014585127 @default.
• W1992639306 cites W2015788732 @default.
• W1992639306 cites W2016433547 @default.
• W1992639306 cites W2017459569 @default.
• W1992639306 cites W2018978482 @default.
• W1992639306 cites W2019937643 @default.
• W1992639306 cites W2025860749 @default.
• W1992639306 cites W2026367990 @default.
• W1992639306 cites W2027839510 @default.
• W1992639306 cites W2033650768 @default.
• W1992639306 cites W2036100925 @default.
• W1992639306 cites W2037435728 @default.
• W1992639306 cites W2039281725 @default.
• W1992639306 cites W2043588437 @default.
• W1992639306 cites W2055730141 @default.
• W1992639306 cites W2055734273 @default.
• W1992639306 cites W2069860065 @default.
• W1992639306 cites W2071200462 @default.
• W1992639306 cites W2073811072 @default.
• W1992639306 cites W2074166682 @default.
• W1992639306 cites W2077856033 @default.
• W1992639306 cites W2079599543 @default.
• W1992639306 cites W2080570756 @default.
• W1992639306 cites W2083069925 @default.
• W1992639306 cites W2088285235 @default.
• W1992639306 cites W2093070562 @default.
• W1992639306 cites W2101823052 @default.
• W1992639306 cites W2111862607 @default.
• W1992639306 cites W2115680008 @default.
• W1992639306 cites W2122160136 @default.
• W1992639306 cites W2126940622 @default.
• W1992639306 cites W2129002143 @default.
• W1992639306 cites W2136422599 @default.
• W1992639306 cites W2146130931 @default.
• W1992639306 cites W2146961403 @default.
• W1992639306 cites W2160483038 @default.
• W1992639306 cites W2161539096 @default.
• W1992639306 cites W2314330139 @default.
• W1992639306 cites W2436735992 @default.
• W1992639306 cites W4230131399 @default.
• W1992639306 cites W4245011475 @default.
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• W1992639306 doi "https://doi.org/10.1016/j.neuroscience.2006.01.019" @default.
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