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- W1992643151 abstract "The effects on tissue fine structure of variations in the colloid osmotic pressure of fixative solutions used for perfusion fixation were studied. Groups of rats were perfusion-fixed with glutaraldehyde solutions which varied only with respect to their concentration of dextran or polyvinylpyrrolidone (PVP). Tissues from several organs, including kidney, pancreas, and intestine, were analyzed by light and electron microscopy. In all organs there were distinct differences in structure between tissues fixed with fixatives containing dextran or PVP and those fixed with solutions devoid of macromolecules. Following fixation without macromolecules in the fixative, the extravascular spaces were greatly enlarged in comparison to similar spaces in tissues fixed in an identical way but with dextran or PVP in the fixative. It is proposed that these and other observed differences are due to differences in the colloid osmotic pressure of the fixations. The results indicate that oncotically active substances should be included in aldehyde fixatives used for perfusion, in particular when structures or cellular relationships in labile extravascular spaces are studied." @default.
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- W1992643151 title "Effects on tissue fine structure of variations in colloid osmotic pressure of glutaraldehyde fixatives" @default.
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- W1992643151 doi "https://doi.org/10.1016/s0022-5320(70)90073-0" @default.
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