FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992661034> ?p ?o ?g. }

• W1992661034 endingPage "894" @default.
• W1992661034 startingPage "879" @default.
• W1992661034 abstract "Solvational perturbations, accomplished by the addition of the three model cosolvents glycerol, ethanol and trifluoroethanol, exert pronounced and diversified effects on the unfolding, non-native assembly and fibril formation of the amyloidogenic protein insulin. Fluorescence, CD and UV-spectroscopic methods as well as atomic force microscopy imaging have been employed to reveal distinct structural and kinetic features upon the aggregation of insulin under different solvational perturbations, which ultimately manifest in morphological variations of mature aggregates and fibrils. In particular, fluorescence anisotropy studies proved to be very valuable in characterizing the corresponding aggregation nuclei. Glycerol stabilizes, through enhanced hydration, native oligomerization and retards fibrillar aggregation at all concentrations studied (up to 40% (w/w)). In contrast, both monoalcohols facilitate the formation of aggregation-prone intermediates by destabilization of the native assembly. The reversal from a kosmotropic to a merely chaotropic solvational behaviour can explain the accelerating effect on ordered fibrillation of low concentrations and the inhibitory nature of high concentrations of ethanol and trifluoroethanol, ultimately leading to amorphous aggregate structures. Mechanistically, dimer dissociation under stabilizing and nucleation under destabilizing conditions have been identified to be the rate-limiting steps that account for the non-monotonic concentration effects of the monoalcohols on the aggregation kinetics. A rationale as to how solvational constraints can tune the stability of the species on the native self-assembly and non-native aggregation pathway, and the energetic barriers that need to be overcome for the required structural interconversions has been put forward. We may propose that the concept of perturbed solvation is generally applicable to phenomena that are related to pathogenic amyloidogenesis of proteins and, in general, solvational effects, besides other aspects of the cellular environment, may play a significant role in a reshaping of the folding/aggregation funnel of proteins." @default.
• W1992661034 created "2016-06-24" @default.
• W1992661034 creator A5028928403 @default.
• W1992661034 creator A5032056243 @default.
• W1992661034 creator A5049297420 @default.
• W1992661034 date "2005-08-01" @default.
• W1992661034 modified "2023-10-16" @default.
• W1992661034 title "Solvational Tuning of the Unfolding, Aggregation and Amyloidogenesis of Insulin" @default.
• W1992661034 cites W1562088891 @default.
• W1992661034 cites W1964161207 @default.
• W1992661034 cites W1965156062 @default.
• W1992661034 cites W1966771308 @default.
• W1992661034 cites W1979509752 @default.
• W1992661034 cites W1980125299 @default.
• W1992661034 cites W1980752153 @default.
• W1992661034 cites W1981131995 @default.
• W1992661034 cites W1984729685 @default.
• W1992661034 cites W1988089792 @default.
• W1992661034 cites W1991347541 @default.
• W1992661034 cites W1992473287 @default.
• W1992661034 cites W1995247791 @default.
• W1992661034 cites W1998205923 @default.
• W1992661034 cites W2001363066 @default.
• W1992661034 cites W2003129326 @default.
• W1992661034 cites W2004470794 @default.
• W1992661034 cites W2006570293 @default.
• W1992661034 cites W2009890105 @default.
• W1992661034 cites W2010462701 @default.
• W1992661034 cites W2014571034 @default.
• W1992661034 cites W2020215335 @default.
• W1992661034 cites W2024683933 @default.
• W1992661034 cites W2024895112 @default.
• W1992661034 cites W2027616961 @default.
• W1992661034 cites W2035625594 @default.
• W1992661034 cites W2039873895 @default.
• W1992661034 cites W2040662242 @default.
• W1992661034 cites W2040824780 @default.
• W1992661034 cites W2043750499 @default.
• W1992661034 cites W2045916983 @default.
• W1992661034 cites W2050079896 @default.
• W1992661034 cites W2050973309 @default.
• W1992661034 cites W2053950670 @default.
• W1992661034 cites W2055307767 @default.
• W1992661034 cites W2059804089 @default.
• W1992661034 cites W2060495639 @default.
• W1992661034 cites W2061734511 @default.
• W1992661034 cites W2066506744 @default.
• W1992661034 cites W2072792448 @default.
• W1992661034 cites W2075584456 @default.
• W1992661034 cites W2075954862 @default.
• W1992661034 cites W2076834744 @default.
• W1992661034 cites W2086924272 @default.
• W1992661034 cites W2088092019 @default.
• W1992661034 cites W2096425372 @default.
• W1992661034 cites W2099092270 @default.
• W1992661034 cites W2101614182 @default.
• W1992661034 cites W2104940502 @default.
• W1992661034 cites W2112622637 @default.
• W1992661034 cites W2114668205 @default.
• W1992661034 cites W2118864235 @default.
• W1992661034 cites W2134661315 @default.
• W1992661034 cites W2136163423 @default.
• W1992661034 cites W2138531756 @default.
• W1992661034 cites W2145589684 @default.
• W1992661034 cites W2149138461 @default.
• W1992661034 cites W2163895770 @default.
• W1992661034 cites W2329618097 @default.
• W1992661034 cites W2593211299 @default.
• W1992661034 cites W4211239749 @default.
• W1992661034 cites W84506637 @default.
• W1992661034 doi "https://doi.org/10.1016/j.jmb.2005.06.046" @default.
• W1992661034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16051271" @default.
• W1992661034 hasPublicationYear "2005" @default.
• W1992661034 type Work @default.
• W1992661034 sameAs 1992661034 @default.
• W1992661034 citedByCount "91" @default.
• W1992661034 countsByYear W19926610342012 @default.
• W1992661034 countsByYear W19926610342013 @default.
• W1992661034 countsByYear W19926610342014 @default.
• W1992661034 countsByYear W19926610342015 @default.
• W1992661034 countsByYear W19926610342016 @default.
• W1992661034 countsByYear W19926610342017 @default.
• W1992661034 countsByYear W19926610342019 @default.
• W1992661034 countsByYear W19926610342020 @default.
• W1992661034 countsByYear W19926610342021 @default.
• W1992661034 countsByYear W19926610342022 @default.
• W1992661034 countsByYear W19926610342023 @default.
• W1992661034 crossrefType "journal-article" @default.
• W1992661034 hasAuthorship W1992661034A5028928403 @default.
• W1992661034 hasAuthorship W1992661034A5032056243 @default.
• W1992661034 hasAuthorship W1992661034A5049297420 @default.
• W1992661034 hasConcept C102362939 @default.
• W1992661034 hasConcept C121332964 @default.
• W1992661034 hasConcept C12554922 @default.
• W1992661034 hasConcept C136238340 @default.
• W1992661034 hasConcept C148898269 @default.
• W1992661034 hasConcept C178790620 @default.
• W1992661034 hasConcept C185592680 @default.

• next